Keywords:
Neural crest.
;
Wnt Proteins--physiology.
;
Neural Crest--physiology.
;
Somites--physiology.
;
Somite.
;
Wnt proteins.
;
Electronic books.
Type of Medium:
Online Resource
Pages:
1 online resource (74 pages)
Edition:
1st ed.
ISBN:
9783540777274
Series Statement:
Advances in Anatomy, Embryology and Cell Biology Series ; v.195
URL:
https://ebookcentral.proquest.com/lib/geomar/detail.action?docID=372965
DDC:
573.86387
Language:
English
Note:
Intro -- CONTENTS -- 1 Introduction -- 1.1 Wnts and Development -- 1.2 Somite Formation -- 1.3 Induction, Delamination and Migration of Neural Crest -- 1.3.1 Induction of the Neural Crest -- 1.3.2 Delamination and Migration of the Neural Crest -- 1.4 Outline of Research Plan -- 1.4.1 Wnt6 and Somitogenesis -- 1.4.2 The Regulation of cNkd1 Expression -- 1.4.3 Wnt6 and Neural Crest -- 2 Materials and Methods -- 2.1 Embryos -- 2.2 Culture of Wnt-Expressing Cells -- 2.3 Operations -- 2.4 BrdU Experiment -- 2.5 Neural Crest Cell Culture -- 2.6 HNK Staining Neural Crest Culture -- 2.7 Wnt6 siRNA RCAS Construct -- 2.8 Immunohistochemistry on Sections -- 2.9 Immunohistochemistry on Whole Embryos -- 2.10 Whole-Mount In Situ Hybridisation -- 2.11 Chemicals -- 2.12 Anti-sense RNA Probes -- 2.12.1 DNA Linearisation -- 2.12.2 DNA Purification -- 2.12.3 Probe Synthesis -- 3 Results -- 3.1 Wnt6 and Somitogenesis -- 3.1.1 Consequences of Ectoderm Removal on the Epithelialisation of Segmental Plate -- 3.1.2 Effect of Wnt6 on the Development of Somites -- 3.1.3 Wnt6 Rescues the Effect of Ectoderm Removal -- 3.1.4 Wnt6 Rescues the Effect of Medial Barrier Insertion -- 3.1.5 Effect of Wnt6 Expression on Muscle Development -- 3.1.6 Effect of Sfrp2 on Somite Development -- viii List of Contents 3.2 The Regulation of cNkd1 Expression -- 3.2.1 Normal Expression of cNkd1 in Chicken Embryos from HH8-HH20 -- 3.2.2 Regulation of cNkd1 Expression -- 3.2.3 Regulation of cNkd1 by Members of the Wnt Family -- 3.3 Wnt6 and Neural Crest -- 3.3.1 Wnt6 Induces Neural Crest Production and Wnt1 Inhibits Neural Crest Induction -- 3.3.2 Wnt6 Induces Neural Crest Production Through the Non-canonical Signalling Pathway -- 3.3.3 Activation of the Non-canonical Pathway Through Dsh Induces Neural Crest -- 3.3.4 The Canonical Wnt Pathway Inhibits Neural Crest Induction.
,
3.3.5 Specific Inhibition of Wnt6 Reduces Neural Crest Production -- 4 Discussion -- 4.1 Wnt6 and Somitogenesis -- 4.2 The Regulation of cNkd1 Expression -- 4.3 Wnts and Neural Crest Induction -- References.
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