Keywords:
Embryology, Experimental -- Laboratory manuals.
;
Embryology, Human -- Laboratory manuals.
;
Electronic books.
Description / Table of Contents:
Never before has there been such a comprehensive book of protocols. This compendium offers a full range of research techniques-from cell culture, to biochemical, to microscopic and genetic. More focused books, like Cold Spring Harbor's Manipulating the Mouse Embryo, are similar though more narrow in scope. This book will appeal to a broad range of researchers, from basic experimental scientists to clinical and animal scientists.
Type of Medium:
Online Resource
Pages:
1 online resource (409 pages)
Edition:
1st ed.
ISBN:
9780198032519
URL:
https://ebookcentral.proquest.com/lib/geomar/detail.action?docID=279527
DDC:
571.8619
Language:
English
Note:
Intro -- Contents -- Contributors -- Abbreviations -- 1. Follicle Development in Vitro -- 2. Preparation of Gametes, in Vitro Maturation, in Vitro Fertilization, and Embryo Recovery and Transfer -- 3. Culture of the Mammalian Preimplantation Embryo -- 4. Assessment of Preimplantation Embryo Development and Viability -- 5. Micromanipulation of Gametes -- 6. Micromanipulation: Biopsy -- 7. Analysis of Intracellular Ions in Embryos: pH and Calcium -- 8. Assessment of Nutrient Uptake, Metabolite Production, and Enzyme Activity -- 9. Metabolic Pathway Activity -- 10. Confocal Imaging of Structural Molecules in Mammalian Gametes -- 11. Fluorescent in situ Hybridisation for Detection of Aneuploidy in Single Human Blastomeres for Preimplantation Genetic Diagnosis -- 12. Assessment of Ploidy, Telomere Length, and Telomerase Activity in Oocytes and Embryos -- 13. Microscale RNA Isolation from Mammalian Embryos -- 14. Relative mRNA Transcript Abundance in Early Embryos by Reverse Transcription-Polymerase Chain Reaction -- 15. Characterization of Novel Genes during Early Development by Application of Differential Display RT-PCR -- 16. Two-Dimensional Protein Gel Database Analysis of Embryos, Oocytes, and Oocyte-Associated Granulosa Cells -- 17. Antisense Disruption of Gene Expression in the Preimplantation Embryo -- 18. Gene Subtraction and Analysis -- 19. Analysis of Apoptosis in the Preimplantation Embryo -- 20. Cytoplasmic Signaling and Cell Cycle Control in the Mouse Egg and Embryo -- 21. Human Embryonic Stem Cells -- 22. Spermatogonial Transplantation -- 23. Somatic Cell Nuclear Transfer -- 24. Cryobiology: Slow Freezing Vitrification of Embryos -- Index -- A -- B -- C -- D -- E -- F -- G -- H -- I -- K -- L -- M -- N -- O -- P -- Q -- R -- S -- T -- U -- V -- Z.
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