GLORIA

GEOMAR Library Ocean Research Information Access

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Copper Coordination Compounds of Polyimidazole-Thioether Ligands. X-ray Structures and Characterization of {Bis[4-((methylthio)ethyl)imidazol-2-yl]methane}bis(tetrafluoroborato)copper(II), Dichloro{(imidazol-2-yl)[4-((methylthio)ethyl)imidazol-2-yl]methane}copper(II), and {[4-(imidazol-2-ylmethyl)imidazol-2-yl][4-((methylthio)ethyl)imidazol-2-yl]methane}bis(perchlorato)copper(II) (1994)
    s.l. : American Chemical Society
    Inorganic chemistry 33 (1994), S. 2808-2814 
    Details
    ISSN: 1520-510X
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ic00091a022
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Calculated Plasma Parameters and Excitation Spectra of High-Pressure Helium Discharges (1999)
    Springer
    Plasma chemistry and plasma processing 19 (1999), S. 467-486 
    Details
    ISSN: 1572-8986
    Keywords: He-plasma ; excitation spectra ; modeling ; comparison with experiments
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Technology
    Notes: Abstract A collisional-radiative model was used to study the kinetics of an atmospheric pressure helium discharge. The electron kinetics was obtained from a two-term solution of the Boltzmann equation with electron–electron collisions included. The distribution of the helium electronic excited states was compared to measured values and used to calculate excitation temperatures. The results show that a unique value of the excitation temperature cannot be used to characterize the whole electronic states distribution, because the plasma is not in local thermodynamical equilibrium under the conditions considered. Other calculated discharge parameters, such as the electron temperature, the maintenance electric field, the density of metastable atoms in the 2 3 S state, and the ion densities are presented and compared to experimental data when available.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1021878309471
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Numerical Modeling of a He–N2 Capillary Surface Wave Discharge at Atmospheric Pressure (2000)
    Springer
    Plasma chemistry and plasma processing 20 (2000), S. 183-207 
    Details
    ISSN: 1572-8986
    Keywords: collisional-radiative model ; He–N2 plasma ; Boltzmann equation ; atmospheric pressure plasma ; surface wave
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Technology
    Notes: Abstract A self-consistent steady-state spatially averaged collisional-radiativemodel in which the rate coefficients involving electrons are calculatedfrom the solution to the electron Boltzmann equation has been developedfor describing an atmospheric pressure plasma in helium–nitrogen(He–N2) mixtures. The influence of small nitrogenconcentrations (typically less than 1%) on the discharge characteristicsis studied and compared with available experimental data. It is foundthat nitrogen is highly dissociated and that the density of metastablehelium atoms is considerably reduced by the presence of nitrogen, evenat such low concentrations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007065022725
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Biological mineralization of iron: Studies using Mössbauer spectroscopy and complementary techniques (1988)
    Springer
    Hyperfine interactions 40 (1988), S. 223-233 
    Details
    ISSN: 1572-9540
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Biological deposition of solid Fe-containing phases can be studied using57Fe Mössbauer spectroscopy. Other techniques are needed in order to understand this complex process. These include proton-induced X-ray and γ-ray emission (PIXE/PIGME), electron microscopy, electron and X-ray diffraction, infrared spectroscopy and chemical characterization of organic components. This paper reviews and evaluates the application of these techniques to biological mineralization of Fe, particularly that occurring in the radula teeth of the marine molluscs, chitons and limpets.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02049092
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Core structures of haemosiderins deposited in various organs in β-thalassaemia/haemoglobin e disease (1992)
    Springer
    Hyperfine interactions 71 (1992), S. 1279-1282 
    Details
    ISSN: 1572-9540
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Mössbauer spectra were recorded of tissue from β-thalassaemia/haemoglobin E spleen, liver, pancreas and heart and of crude haemosiderins (insoluble iron fractions) isolated from the organs. Iron in the crude haemosiderins from the spleen and heart remains paramagnetic below 4.2K indicating that the iron is in a non-crystalline form. Superparamagnetic behaviour of the crude haemosiderins from the pancreas and liver indicate the presence of ferrihydrite cores with some cores with a structure based on defect-goethite.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02397317
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Atomic spectroscopy with surface wave plasmas (1996)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 355 (1996), S. 494-500 
    Details
    ISSN: 1618-2650
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The use of microwave induced plasmas, particularly of surface wave plasmas, as detectors in atomic emission spectrometry for elemental analysis is reviewed. Surface wave plasmas have been produced at low HF power and used as gas chromatographic detectors. The analytical performances for the detection of non-metals with a Fourier transform spectrometer and a two-channel filter unit are reported. The excitation behavior of non-metals in helium-based mixed gas-plasmas has also be studied. In particular, the effect of power and of nitrogen concentration on the bromine emission has been systematically investigated. A nine-fold improvement of the detection limits for bromine can be obtained in a high power (900 W) helium-nitrogen (0.1–0.2%) plasma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s0021663550494
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    facet.materialart.
    Unknown
    The Interactome of the Human Respiratory Syncytial Virus NS1 Protein Highlights Multiple Effects on Host Cell Biology [Virus-Cell Interactions] (2012)
    The American Society for Microbiology (ASM)
    Details
    Publication Date: 2012-07-12
    Description: Viral proteins can have multiple effects on host cell biology. Human respiratory syncytial virus (HRSV) nonstructural protein 1 (NS1) is a good example of this. During the virus life cycle, NS1 can act as an antagonist of host type I and III interferon production and signaling, inhibit apoptosis, suppress dendritic cell maturation, control protein stability, and regulate transcription of host cell mRNAs, among other functions. It is likely that NS1 performs these different roles through interactions with multiple host cell proteins. To investigate this and identify cellular proteins that could interact with NS1, we used quantitative proteomics in combination with green fluorescent protein (GFP)-trap immunoprecipitation and bioinformatic analysis. This analysis identified 221 proteins that were potentially part of complexes that could interact with NS1, with many of these associated with transcriptional regulation as part of the mediator complex, cell cycle regulation, and other functions previously assigned to NS1. Specific immunoprecipitation using the GFP trap was used to confirm the ability of selected cellular proteins to interact individually with NS1. Infection of A549 cells with recombinant viruses deficient in the expression of NS1 and overexpression analysis both demonstrated that NS1 was necessary and sufficient for the enrichment of cells in the G 1 phase of the cell cycle.
    Print ISSN: 0022-538X
    Electronic ISSN: 1098-5514
    Topics: Medicine
    Published by The American Society for Microbiology (ASM)
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    PAPER CURRENT
  • 8
    facet.materialart.
    Unknown
    Identification of Respiratory Syncytial Virus Nonstructural Protein 2 Residues Essential for Exploitation of the Host Ubiquitin System and Inhibition of Innate Immune Responses [Cellular Response to Infection] (2016)
    The American Society for Microbiology (ASM)
    Details
    Publication Date: 2016-06-25
    Description: Respiratory syncytial virus (RSV) is a leading cause of lower respiratory tract infection in young children worldwide. The RSV nonstructural protein 2 (NS2) is a multifunctional protein that primarily acts to antagonize the innate immune system by targeting STAT2 for proteasomal degradation. We investigated the structural determinants of NS2 important for interaction with the host ubiquitin system to degrade STAT2 during infection. We found that NS2 expression enhances ubiquitination of host proteins. Bioinformatics analysis provided a platform for identification of specific residues that limit NS2-induced ubiquitination. Combinations of multiple mutations displayed an additive effect on reducing NS2-induced ubiquitination. Using a reverse genetics system, we generated recombinant RSV (rRSV) containing NS2 ubiquitin mutations, which maintained their effect on ubiquitin expression during infection. Interestingly, STAT2 degradation activity was ablated in the NS2 ubiquitin mutant rRSV. In addition, NS2 ubiquitin mutations decreased rRSV replication, indicating a correlation between NS2's ubiquitin function and antagonism of innate immune signaling to enhance viral replication. Our approach of targeting NS2 residues required for NS2 inhibition of immune responses provides a mechanism for attenuating RSV for vaccine development. IMPORTANCE RSV has been circulating globally for more than 60 years, causing severe respiratory disease in pediatric, elderly, and immunocompromised populations. Production of a safe, effective vaccine against RSV is a public health priority. The NS2 protein is an effective target for prevention and treatment of RSV due to its antagonistic activity against the innate immune system. However, NS2-deleted RSV vaccine candidates rendered RSV overattenuated or poorly immunogenic. Alternatively, we can modify essential NS2 structural features to marginally limit viral growth while maintaining immune responses, providing the necessary balance between antigenicity and safety required for an effective vaccine. We coupled bioinformatics analysis with reverse genetics to introduce mutations into RSV's negative-sense genome. In this way we constructed rRSV NS2 ubiquitin mutants that limited NS2's ability to antagonize the innate immune system, thereby attenuating rRSV growth and increasing innate immune responses.
    Print ISSN: 0022-538X
    Electronic ISSN: 1098-5514
    Topics: Medicine
    Published by The American Society for Microbiology (ASM)
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    PAPER CURRENT
  • 9
    facet.materialart.
    Unknown
    The Thr205 Phosphorylation Site within Respiratory Syncytial Virus Matrix (M) Protein Modulates M Oligomerization and Virus Production [Structure and Assembly] (2014)
    The American Society for Microbiology (ASM)
    Details
    Publication Date: 2014-05-07
    Description: Human respiratory syncytial virus (RSV) is the most common cause of bronchiolitis and pneumonia in infants and the elderly worldwide; however, there is no licensed RSV vaccine or effective drug treatment available. The RSV matrix (M) protein plays key roles in virus assembly and budding, but the protein interactions that govern budding of infectious virus are not known. In this study, we focus on M protein and identify a key phosphorylation site (Thr205) in M that is critical for RSV infectious virus production. Recombinant virus with a nonphosphorylatable alanine (Ala) residue at the site was markedly attenuated, whereas virus with a phosphomimetic aspartate (Asp) resulted in a nonviable virus which could only be recovered with an additional mutation in M (serine to asparagine at position 220), strongly implying that Thr205 is critical for viral infectivity. Experiments in vitro showed that mutation of Thr205 does not affect M stability or the ability to form dimers but implicate an effect on higher-order oligomer assembly. In transfected and infected cells, Asp substitution of Thr205 appeared to impair M oligomerization; typical filamentous structures still formed at the plasma membrane, but M assembly during the ensuing elongation process seemed to be impaired, resulting in shorter and more branched filaments as observed using electron microscopy (EM). Our data thus imply for the first time that M oligomerization, regulated by a negative charge at Thr205, may be critical to production of infectious RSV. IMPORTANCE We show here for the first time that RSV M's role in virus assembly/release is strongly dependent on threonine 205 (Thr205), a consensus site for CK2, which appears to play a key regulatory role in modulating M oligomerization and association with virus filaments. Our analysis indicates that T205 mutations do not impair M dimerization or viruslike filament formation per se but rather the ability of M to assemble in ordered fashion on the viral filaments themselves. This appears to impact in turn upon the infectivity of released virus rather than on virus production or release itself. Thus, M oligomerization would appear to be a target of interest for the development of anti-RSV agents; further, the recombinant T205-substituted mutant viruses described here would appear to be the first RSV mutants affected in viral maturation to our knowledge and hence of considerable interest for vaccine approaches in the future.
    Print ISSN: 0022-538X
    Electronic ISSN: 1098-5514
    Topics: Medicine
    Published by The American Society for Microbiology (ASM)
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    PAPER CURRENT
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...