GLORIA

GEOMAR Library Ocean Research Information Access

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Diel vertical migrations ofMeganyctiphanes norvegica in the Kattegat: Comparison of net catches and measurements with Acoustic Doppler Current Profilers (1995)
    Springer
    Helgoland marine research 49 (1995), S. 849-866 
    Details
    ISSN: 1438-3888
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Diel vertical migration of a stable and well-defined population of Nordic krill,Meganyctiphanes norvegica (Crustacea, Euphausiacea) was investigated during eight days in August 1989, in the Läsö-Deep, East of the Danish island Läsö. Net catches with a multi-net (MOCNESS) and measurements with a moored and a shipboard Acoustic Doppler Current Profiler (ADCP) were compared. Backscattered energy as a measure for biomass gave good correlations to the dry weight ofM. norvegica and smaller zooplankton from net catches. Diel migratory patterns matched well, as determined, parallel with both methods. Migratory vertical velocity was determined with ADCP at 2–3 cm sec−1. The potential for the use of ADCPs for biological investigation is discussed. Vertical migration was dependent on environmental parameters. The krill did not cross a temperature barrier of 14°C, although rich food sources were situated beyond it. Differences in salinity did not play a role. Currents were involved in plankton distribution. Light was an important Zeitgeber (synchronizer) and determined the density of the krill aggregations. Feeding behaviour did not interfere with the light-induced migratory pattern of Nordic krill at the Läsö-Deep.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02368407
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Colonization behaviour of two enterobacterial strains on cereals (2000)
    Springer
    Biology and fertility of soils 30 (2000), S. 550-557 
    Details
    ISSN: 1432-0789
    Keywords: Key words Bacteria-plant interaction ; Pantoea agglomerans ; Klebsiella pneumoniae ; Double antibody sandwich enzyme linked immunosorbent assay ; Population densities
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  Two diazotrophic enterobacterial strains, Pantoea agglomerans D5/23 and Klebsiella pneumoniae CC12/12, were observed in sterile and non-sterile hydroponic and soil experiments in order to determine, by means of an immunological detection method (double antibody sandwich enzyme linked immunosorbent assay), their colonization sites, their migration within individuals of different plant species, and their ability to compete with indigenous organisms. To investigate the interaction between bacteria and plants, root and shoot samples were analysed using electron microscopy. Field experiments were performed to determine the growth-promoting abilities of the bacterial strains. In field experiments, inoculation with P. agglomerans led to an increase in the grain yield of different wheat (Triticum aestivum) cultivars. The same strain was also able to colonize the rhizosphere and the phyllosphere of different cereals due to its ability to migrate within the plant. Roots and media were colonized 10–100 times more intensively than shoots, with about 106 cells g–1 root and 104 cells g–1 shoot. We found that P. agglomerans colonized the root and plant-growth medium of wheat to a greater extent than those of rye (Secale cereale) and barley (Hordeum vulgare), whereas the colonization of shoots was higher in rye and barley compared to wheat. Furthermore, while cell numbers of K. pneumoniae in media and roots were 10 times higher than cell numbers of P. agglomerans, only the latter markedly increased root growth. We were able to detect significant differences in colonization numbers between treatments even if the data were not normally or log-normally distributed or the variances were not homogenous.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003740050035
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Ultrastructure of the integument of a pelagic Crustacean: moult cycle related studies on the Antarctic krill, Euphausia superba (1989)
    Springer
    Marine biology 101 (1989), S. 355-365 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The ultrastructure of euphausiid integument was examined in relation to the moult cycle and supplemented by investigations of chitinase activity in the integument and content of N-acetyl-β-D-glucosamine in the hemolymph. The Antarctic krill, Euphausia superba was collected in 1983 in Admiralty Bay, King George Island, Antarctica. Some specimens of the Northern krill, Meganyctiphanes norvegica, from the Danish Kattegat served for comparison. As a major aim of the study, the moult staging system developed for living tissue could be verified by ultrastructural findings. Under experimental high production conditions of the Antarctic summer, no period of rest or “intermoult” between post- and premoult was observed in subadult E. superba. Neither was a resting phase seen at the cellular level, the epidermis remained active. The epidermal gland cells did not show any cyclical changes, and the organelles of protein synthesis were generally well developed in all moult stages. In order to follow the physiological course of events, structural and biochemical methods were combined and showed as a result that the last moult stage before ecdysis is characterized by massive cuticular resorption. The epicuticle remained ultrastructurally unchanged before and after ecdysis, even though its permeability should alter at ecdysis. The existence of muscle insertions which connect the old and the new cuticle across the exuvial space suggests an answer to the question why E. superba is hardly impaired in swimming almost up to the time of ecdysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00428132
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    facet.materialart.
    Unknown
    SEAMOX: The Influence of Seamounts and Oxygen Minimum Zones on Pelagic Fauna in the Eastern Tropical Atlantic Cruise No. MSM49 November 28 - December 21, 2015 Las Palmas de Gran Canaria (Spain) - Mindelo (Republic of Cape Verde) (2016)
    DFG-Senatskommission für Ozeanographie
    In:  Maria S. Merian-Berichte, MSM49 . DFG-Senatskommission für Ozeanographie, Bremen, Germany, 42 pp.
    Details
    Publication Date: 2019-09-23
    Type: Report , NonPeerReviewed
    Format: text
    DOI: 10.2312/cr_msm49
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    OceanRep: Report - Cruise Report
  • 5
    facet.materialart.
    Unknown
    The Receptor Attachment Function of Measles Virus Hemagglutinin Can Be Replaced with an Autonomous Protein That Binds Her2/neu While Maintaining Its Fusion-Helper Function [Gene Delivery] (2013)
    The American Society for Microbiology (ASM)
    Details
    Publication Date: 2013-05-08
    Description: Cell entry of enveloped viruses is initiated by attachment to the virus receptor followed by fusion between the virus and host cell membranes. Measles virus (MV) attachment to its receptor is mediated by the hemagglutinin (H), which is thought to produce conformational changes in the membrane fusion protein (F) that trigger insertion of its fusion peptide into the target cell membrane. Here, we uncoupled receptor attachment and the fusion-helper function of H by introducing Y481A, R533A, S548L, and F549S mutations into the viral attachment protein that made it blind to its normal receptors. An artificial receptor attachment protein specific for Her2/ neu was incorporated into the membranes of pseudotyped lentivirus particles as a separate transmembrane protein along with the F protein. Surprisingly, these particles entered efficiently into Her2/ neu -positive SK-OV-3 as well as CHO-Her2 cells. Cell entry was independent of endocytosis but strictly dependent on the presence of H. H-specific monoclonal antibodies, as well as a mutation in H interfering with H/F cooperation, blocked cell entry. The particles mediated stable and specific transfer of reporter genes into Her2/ neu -positive human tumor cells also in vivo , while exhibiting improved infectivity and higher titers than Her2/ neu -targeted vectors displaying the targeting domain on H. Extending the current model of MV cell entry, the data suggest that receptor binding of H is not required for its fusion-helper function but that particle-cell contact in general may be sufficient to induce the conformational changes in the H/F complex and activate membrane fusion.
    Print ISSN: 0022-538X
    Electronic ISSN: 1098-5514
    Topics: Medicine
    Published by The American Society for Microbiology (ASM)
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    PAPER CURRENT
  • 6
    facet.materialart.
    Unknown
    Exclusive Transduction of Human CD4+ T Cells upon Systemic Delivery of CD4-Targeted Lentiviral Vectors [NOVEL IMMUNOLOGICAL METHODS] (2015)
    The American Association of Immunologists (AAI)
    Details
    Publication Date: 2015-08-22
    Description: Playing a central role in both innate and adaptive immunity, CD4 + T cells are a key target for genetic modifications in basic research and immunotherapy. In this article, we describe novel lentiviral vectors (CD4-LV) that have been rendered selective for human or simian CD4 + cells by surface engineering. When applied to PBMCs, CD4-LV transduced CD4 + but not CD4 – cells. Notably, also unstimulated T cells were stably genetically modified. Upon systemic or intrasplenic administration into mice reconstituted with human PBMCs or hematopoietic stem cells, reporter gene expression was predominantly detected in lymphoid organs. Evaluation of GFP expression in organ-derived cells and blood by flow cytometry demonstrated exclusive gene transfer into CD4 + human lymphocytes. In bone marrow and spleen, memory T cells were preferentially hit. Toward therapeutic applications, we also show that CD4-LV can be used for HIV gene therapy, as well as for tumor therapy, by delivering chimeric Ag receptors. The potential for in vivo delivery of the FOXP3 gene was also demonstrated, making CD4-LV a powerful tool for inducible regulatory T cell generation. In summary, our work demonstrates the exclusive gene transfer into a T cell subset upon systemic vector administration opening an avenue toward novel strategies in immunotherapy.
    Print ISSN: 0022-1767
    Electronic ISSN: 1550-6606
    Topics: Medicine
    Published by The American Association of Immunologists (AAI)
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    PAPER CURRENT
  • 7
    facet.materialart.
    Unknown
    Specific gene delivery to liver sinusoidal and artery endothelial cells (2013)
    American Society of Hematology (ASH)
    In: Blood
    Details
    Publication Date: 2013-09-20
    Description: Different types of endothelial cells (EC) fulfill distinct tasks depending on their microenvironment. ECs are therefore difficult to genetically manipulate ex vivo for functional studies or gene therapy. We assessed lentiviral vectors (LVs) targeted to the EC surface marker CD105 for in vivo gene delivery. The mouse CD105-specific vector, mCD105-LV, transduced only CD105-positive cells in primary liver cell cultures. Upon systemic injection, strong reporter gene expression was detected in liver where mCD105-LV specifically transduced liver sinusoidal ECs (LSECs) but not Kupffer cells, which were mainly transduced by nontargeted LVs. Tumor ECs were specifically targeted upon intratumoral vector injection. Delivery of the erythropoietin gene with mCD105-LV resulted in substantially increased erythropoietin and hematocrit levels. The human CD105-specific vector (huCD105-LV) transduced exclusively human LSECs in mice transplanted with human liver ECs. Interestingly, when applied at higher dose and in absence of target cells in the liver, huCD105-LV transduced ECs of a human artery transplanted into the descending mouse aorta. The data demonstrate for the first time targeted gene delivery to specialized ECs upon systemic vector administration. This strategy offers novel options to better understand the physiological functions of ECs and to treat genetic diseases such as those affecting blood factors.
    Keywords: Vascular Biology, Gene Therapy
    Print ISSN: 0006-4971
    Electronic ISSN: 1528-0020
    Topics: Biology , Medicine
    Published by American Society of Hematology (ASH)
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    PAPER CURRENT
  • 8
    facet.materialart.
    Unknown
    Analysis of charge and orbital order in Fe_{3}O_{4} by Fe L_{2,3} resonant x-ray diffraction (2013)
    American Physical Society (APS)
    Details
    Publication Date: 2013-11-07
    Description: Author(s): A. Tanaka, C. F. Chang, M. Buchholz, C. Trabant, E. Schierle, J. Schlappa, D. Schmitz, H. Ott, P. Metcalf, L. H. Tjeng, and C. Schüßler-Langeheine To elucidate charge and orbital order below the Verwey transition temperature T V ∼125 K, a thin layer of magnetite partially detwined by growth on the stepped MgO(001) substrate has been studied by means of soft x-ray diffraction at the Fe L 2,3 resonance. The azimuth angle, incident photon polarizati... [Phys. Rev. B 88, 195110] Published Wed Nov 06, 2013
    Keywords: Electronic structure and strongly correlated systems
    Print ISSN: 1098-0121
    Electronic ISSN: 1095-3795
    Topics: Physics
    Published by American Physical Society (APS)
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    PAPER CURRENT
  • 9
    facet.materialart.
    Unknown
    Symmetry of Orbital Order in Fe_{3}O_{4} Studied by Fe L_{2,3} Resonant X-Ray Diffraction (2012)
    American Physical Society (APS)
    Details
    Publication Date: 2012-05-31
    Description: Author(s): A. Tanaka, C. F. Chang, M. Buchholz, C. Trabant, E. Schierle, J. Schlappa, D. Schmitz, H. Ott, P. Metcalf, L. H. Tjeng, and C. Schüßler-Langeheine We studied the symmetry of the Fe 3 d wave function in magnetite below the Verwey temperature T V with resonant soft-x-ray diffraction. Although the lattice structure of the low-temperature phase of Fe 3 O 4 is well described by the pseudo-orthorhombic P m c a with a slight monoclinic P 2/ c distortion, we fi... [Phys. Rev. Lett. 108, 227203] Published Wed May 30, 2012
    Keywords: Condensed Matter: Electronic Properties, etc.
    Print ISSN: 0031-9007
    Electronic ISSN: 1079-7114
    Topics: Physics
    Published by American Physical Society (APS)
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    PAPER CURRENT
  • 10
    facet.materialart.
    Unknown
    Targeting CD133+ Tumor Cells (2013)
    The American Association for Cancer Research (AACR)
    Details
    Publication Date: 2013-01-18
    Description: Tumor-initiating cells (TIC) are critical yet evasive targets for the development of more effective antitumoral strategies. The cell surface marker CD133 is frequently used to identify TICs of various tumor entities, including hepatocellular cancer and glioblastoma. Here, we describe oncolytic measles viruses (MV) retargeted to CD133. The viruses, termed MV-141.7 and MV-AC133, infected and selectively lysed CD133+ tumor cells. Both viruses exerted strong antitumoral effects on human hepatocellular carcinoma growing subcutaneously or multifocally in the peritoneal cavity of nonobese diabetic/severe combined immunodeficient (NOD/SCID) mice. Notably, the CD133-targeted viruses were more effective in prolonging survival than the parental MV-NSe, which is currently assessed as oncolytic agent in clinical trials. Interestingly, target receptor overexpression or increased spreading kinetics through tumor cells were excluded as being causative for the enhanced oncolytic activity of CD133-targeted viruses. MV-141.7 was also effective in mouse models of orthotopic glioma tumor spheres and primary colon cancer. Our results indicate that CD133-targeted measles viruses selectively eliminate CD133+ cells from tumor tissue, offering a key tool for research in tumor biology and cancer therapy. Cancer Res; 73(2); 865–74. ©2013 AACR.
    Print ISSN: 0008-5472
    Electronic ISSN: 1538-7445
    Topics: Medicine
    Published by The American Association for Cancer Research (AACR)
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    PAPER CURRENT
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...