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  • 1
    Online Resource
    Online Resource
    Lösung der Nährstoff-Ko-Begrenzung der Phytoplankton-Produktivität im äquatorialen Pazifik: Integration von experimentellen, proteomischen und Fluoreszenz-Ansätzen 'eqPac-Co-Limitation' : Schlussbericht (2020)
    Kiel : [GEOMAR Helmholtz Zentrum für Ozeanforschung]
    Details Availability
    Keywords: Forschungsbericht ; Äquatorialpazifik ; Phytoplankton ; Produktivität
    Type of Medium: Online Resource
    Pages: 1 Online-Ressource (16 Seiten, 900,19 KB) , Illustrationen, Diagramme
    URL: https://doi.org/10.2314/KXP:176093657X
    DOI: 10.2314/KXP:176093657X
    Language: German
    Note: Förderkennzeichen BMBF 03G0267TA , Unterschiede zwischen dem gedruckten Dokument und der elektronischen Ressource können nicht ausgeschlossen werden
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  • 2
    Electronic Resource
    Electronic Resource
    Statement on outpatient percutaneous liver biopsy (1989)
    Springer
    Digestive diseases and sciences 34 (1989), S. 322-323 
    Details
    ISSN: 1573-2568
    Keywords: liver biopsy ; guidelines ; outpatient
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This document represents a consensus statement dealing with optimum patient care in a significant clinical area. The statement has been prepared by the Patient Care Committee of the American Gastroenterological Association with the advice of other experts and with peer review. As with all such guidelines, this should be interpreted in a nondogmatic manner, so as not to exclude other therapies or opinions in any particular situation. Based on present knowledge, limited at times, future modifications or other changes in this statement may be necessary.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01536249
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Diagnosis of chest pain of esophageal origin (1990)
    Springer
    Digestive diseases and sciences 35 (1990), S. 289-293 
    Details
    ISSN: 1573-2568
    Keywords: esophagus ; chest pain ; diagnostic evaluation ; noncardiac chest pain
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01537404
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    Paper (German National Licenses)
    Fulltext
  • 4
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    Iron limitation of microbial phosphorus acquisition in the tropical North Atlantic (2017)
    Nature Research
    In:  Nature Communications, 8 (15465).
    Details
    Publication Date: 2021-04-23
    Description: In certain regions of the predominantly nitrogen limited ocean, microbes can become co-limited by phosphorus. Within such regions, a proportion of the dissolved organic phosphorus pool can be accessed by microbes employing a variety of alkaline phosphatase (APase) enzymes. In contrast to the PhoA family of APases that utilize zinc as a cofactor, the recent discovery of iron as a cofactor in the more widespread PhoX and PhoD implies the potential for a biochemically dependant interplay between oceanic zinc, iron and phosphorus cycles. Here we demonstrate enhanced natural community APase activity following iron amendment within the low zinc and moderately low iron Western North Atlantic. In contrast we find no evidence for trace metal limitation of APase activity beneath the Saharan dust plume in the Eastern Atlantic. Such intermittent iron limitation of microbial phosphorus acquisition provides an additional facet in the argument for iron controlling the coupling between oceanic nitrogen and phosphorus cycles.
    Type: Article , PeerReviewed
    Format: text
    Format: text
    DOI: 10.1038/ncomms15465
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    OceanRep: Article in a Scientific Journal - peer-reviewed
  • 5
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    The GEOTRACES Intermediate Data Product 2017 (2018)
    Elsevier
    In:  Chemical Geology, 493 . pp. 210-223.
    Details
    Publication Date: 2021-02-08
    Description: The GEOTRACES Intermediate Data Product 2017 (IDP2017) is the second publicly available data product of the international GEOTRACES programme, and contains data measured and quality controlled before the end of 2016. The IDP2017 includes data from the Atlantic, Pacific, Arctic, Southern and Indian oceans, with about twice the data volume of the previous IDP2014. For the first time, the IDP2017 contains data for a large suite of biogeochemical parameters as well as aerosol and rain data characterising atmospheric trace element and isotope (TEI) sources. The TEI data in the IDP2017 are quality controlled by careful assessment of intercalibration results and multi-laboratory data comparisons at crossover stations. The IDP2017 consists of two parts: (1) a compilation of digital data for more than 450 TEIs as well as standard hydrographic parameters, and (2) the eGEOTRACES Electronic Atlas providing an on-line atlas that includes more than 590 section plots and 130 animated 3D scenes. The digital data are provided in several formats, including ASCII, Excel spreadsheet, netCDF, and Ocean Data View collection. Users can download the full data packages or make their own custom selections with a new on-line data extraction service. In addition to the actual data values, the IDP2017 also contains data quality flags and 1-σ data error values where available. Quality flags and error values are useful for data filtering and for statistical analysis. Metadata about data originators, analytical methods and original publications related to the data are linked in an easily accessible way. The eGEOTRACES Electronic Atlas is the visual representation of the IDP2017 as section plots and rotating 3D scenes. The basin-wide 3D scenes combine data from many cruises and provide quick overviews of large-scale tracer distributions. These 3D scenes provide geographical and bathymetric context that is crucial for the interpretation and assessment of tracer plumes near ocean margins or along ridges. The IDP2017 is the result of a truly international effort involving 326 researchers from 22 countries. This publication provides the critical reference for unpublished data, as well as for studies that make use of a large cross-section of data from the IDP2017.
    Type: Article , PeerReviewed
    Format: text
    DOI: 10.1016/j.chemgeo.2018.05.040
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    OceanRep: Article in a Scientific Journal - peer-reviewed
  • 6
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    Single cell genomes of Prochlorococcus, Synechococcus, and sympatric microbes from diverse marine environments (2018)
    Nature Research
    In:  Scientific Data, 5 . p. 180154.
    Details
    Publication Date: 2021-04-23
    Description: Prochlorococcus and Synechococcus are the dominant primary producers in marine ecosystems and perform a significant fraction of ocean carbon fixation. These cyanobacteria interact with a diverse microbial community that coexists with them. Comparative genomics of cultivated isolates has helped address questions regarding patterns of evolution and diversity among microbes, but the fraction that can be cultivated is miniscule compared to the diversity in the wild. To further probe the diversity of these groups and extend the utility of reference sequence databases, we report a data set of single cell genomes for 489 Prochlorococcus, 50 Synechococcus, 9 extracellular virus particles, and 190 additional microorganisms from a diverse range of bacterial, archaeal, and viral groups. Many of these uncultivated single cell genomes are derived from samples obtained on GEOTRACES cruises and at well-studied oceanographic stations, each with extensive suites of physical, chemical, and biological measurements. The genomic data reported here greatly increases the number of available Prochlorococcus genomes and will facilitate studies on evolutionary biology, microbial ecology, and biological oceanography.
    Type: Article , PeerReviewed
    Format: text
    DOI: 10.1038/sdata.2018.154
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    OceanRep: Article in a Scientific Journal - peer-reviewed
  • 7
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    Determination of picomolar dissolved free amino acids along a South Atlantic transect using reversed-phase high-performance liquid chromatography (2017)
    Elsevier
    In:  Marine Chemistry, 196 . pp. 173-180.
    Details
    Publication Date: 2021-04-23
    Description: Dissolved free amino acids (DFAA) in seawater are a form of nitrogen (N) available for marine microbes. In oligotrophic environments where N-containing nutrients are the limiting factor for microbial growth, N nutrition from DFAA could be crucial, but as yet it is poorly resolved. Measurements of individual DFAA are challenging as concentrations are typically in the low nmol L− 1 range. Here we report modifications to methodology using o-phthaldialdehyde (OPA) derivatization and reversed phase high performance liquid chromatography (HPLC) that provide a 30-fold improvement in sensitivity enabling the detection of 15 amino acids in seawater with a limit of detection as low as 10 pmol L− 1 with accuracy and precision of better than 10%. This analytical methodology is now suitable for the challenging quantitation of DFAA in oligotrophic seawaters. The method was successfully applied to a suite of seawater samples collected on a cruise crossing the South Atlantic Ocean, where concentrations of DFAAs were generally low (sub nmol L− 1), revealing basin-scale features in the oceanographic distributions of DFAA. This unique dataset implies that DFAAs are an important component of the N cycle in both near-coastal and open oceans. Further calculations suggest that the proportions of organic N originating from DFAA sources were significant, contributing between 0.2 and 200% that of NH4 + and up to 77% that of total inorganic nitrogen in the upper 400 m in some regions of the transect.
    Type: Article , PeerReviewed
    Format: text
    Format: text
    DOI: 10.1016/j.marchem.2017.09.008
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    OceanRep: Article in a Scientific Journal - peer-reviewed
  • 8
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    Influence of iron, cobalt, and vitamin B12 supply on phytoplankton growth in the tropical East Pacific during the 2015 El Niño (2018)
    AGU (American Geophysical Union) | Wiley
    In:  Geophysical Research Letters, 45 (12). pp. 6150-6159.
    Details
    Publication Date: 2021-04-23
    Description: Iron (Fe), cobalt (Co), and vitamin B12 addition experiments were performed in the eastern Equatorial Pacific/Peruvian upwelling zone during the 2015 El Niño event. Near the Peruvian coastline, apparent photosystem II photochemical efficiencies (Fv/Fm) were unchanged by nutrient addition and chlorophyll‐a tripled in untreated controls over two days, indicating nutrient replete conditions. Conversely, Fe amendment further away from the coastline in the high nitrate, low Fe zone significantly increased Fv/Fm and chlorophyll‐a concentrations. Mean chlorophyll‐a was further enhanced following supply of Fe+Co and Fe+B12 relative to Fe alone, but this was not statistically significant; further offshore, reported Co depletion relative to Fe could enhance responses. The persistence of Fe limitation in this system under a developing El Niño, as previously demonstrated under non‐El Niño conditions, suggests that diminished upwelled Fe is likely an important factor driving reductions in offshore phytoplankton productivity during these events.
    Type: Article , PeerReviewed
    Format: text
    Format: text
    DOI: 10.1029/2018GL077972
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    OceanRep: Article in a Scientific Journal - peer-reviewed
  • 9
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    Mechanisms of silver nanoparticle toxicity to the coastal marine diatom Chaetoceros curvisetus (2017)
    Nature Research
    In:  Scientific Reports, 7 . Art.Nr. 10777.
    Details
    Publication Date: 2021-04-23
    Description: Inputs of silver nanoparticles (AgNPs) to marine waters continue to increase yet mechanisms of AgNPs toxicity to marine phytoplankton are still not well resolved. This study reports a series of toxicity experiments on a representative coastal marine diatom species Chaetoceros curvisetus using the reference AgNP, NM-300K. Exposure to AgNPs resulted in photosynthetic impairment and loss of diatom biomass in proportion to the supplied AgNP dose. The underlying mechanism of toxicity was explored via comparing biological responses in parallel experiments. Diatom responses to AgNP, free Ag(I) species, and dialysis bag-retained AgNP treatments showed marked similarity, pointing towards a dominant role of Ag(I) species uptake, rather than NPs themselves, in inducing the toxic response. In marked contrast to previous studies, addition of the organic complexing agent cysteine (Cys) alongside Ag only marginally moderated toxicity, implying AgCys− complexes were bioavailable to this diatom species. A preliminary field experiment with a natural phytoplankton community in the southeast Atlantic Ocean showed no significant toxic response at a NM-300 K concentration that resulted in ~40% biomass loss in the culture studies, suggesting a modulating effect of natural seawaters on Ag toxicity.
    Type: Article , PeerReviewed
    Format: text
    Format: text
    DOI: 10.1038/s41598-017-11402-x
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    OceanRep: Article in a Scientific Journal - peer-reviewed
  • 10
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    Nutrient co-limitation at the boundary of an oceanic gyre (2017)
    Nature Research
    In:  Nature, 551 (7679). pp. 242-246.
    Details
    Publication Date: 2021-04-23
    Description: Nutrient limitation of oceanic primary production exerts a fundamental control on marine food webs and the flux of carbon into the deep ocean1. The extensive boundaries of the oligotrophic sub-tropical gyres collectively define the most extreme transition in ocean productivity, but little is known about nutrient limitation in these zones1, 2, 3, 4. Here we present the results of full-factorial nutrient amendment experiments conducted at the eastern boundary of the South Atlantic gyre. We find extensive regions in which the addition of nitrogen or iron individually resulted in no significant phytoplankton growth over 48 hours. However, the addition of both nitrogen and iron increased concentrations of chlorophyll a by up to approximately 40-fold, led to diatom proliferation, and reduced community diversity. Once nitrogen–iron co-limitation had been alleviated, the addition of cobalt or cobalt-containing vitamin B12 could further enhance chlorophyll a yields by up to threefold. Our results suggest that nitrogen–iron co-limitation is pervasive in the ocean, with other micronutrients also approaching co-deficiency. Such multi-nutrient limitations potentially increase phytoplankton community diversity.
    Type: Article , PeerReviewed
    Format: text
    Format: text
    Format: text
    DOI: 10.1038/nature24063
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    OceanRep: Article in a Scientific Journal - peer-reviewed
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