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  • 1
    Publication Date: 2021-02-08
    Description: Climate models project that the Arctic Ocean may experience ice-free summers by the second half of this century. This may have severe repercussions on phytoplankton bloom dynamics and the associated cycling of carbon in surface waters. We currently lack baseline knowledge of the seasonal dynamics of Arctic microbial communities, which is needed in order to better estimate the effects of such changes on ecosystem functioning. Here we present a comparative study of polar summer microbial communities in the ice-free (eastern) and ice-covered (western) hydrographic regimes at the LTER HAUSGARTEN in Fram Strait, the main gateway between the Arctic and North Atlantic Oceans. Based on measured and modeled biogeochemical parameters, we tentatively identified two different ecosystem states (i.e., different phytoplankton bloom stages) in the distinct regions. Using Illumina tag-sequencing, we determined the community composition of both free-living and particle-associated bacteria as well as microbial eukaryotes in the photic layer. Despite substantial horizontal mixing by eddies in Fram Strait, pelagic microbial communities showed distinct differences between the two regimes, with a proposed early spring (pre-bloom) community in the ice-covered western regime (with higher representation of SAR11, SAR202, SAR406 and eukaryotic MALVs) and a community indicative of late summer conditions (post-bloom) in the ice-free eastern regime (with higher representation of Flavobacteria, Gammaproteobacteria and eukaryotic heterotrophs). Co-occurrence networks revealed specific taxon-taxon associations between bacterial and eukaryotic taxa in the two regions. Our results suggest that the predicted changes in sea ice cover and phytoplankton bloom dynamics will have a strong impact on bacterial community dynamics and potentially on biogeochemical cycles in this region.
    Type: Article , PeerReviewed , info:eu-repo/semantics/article
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  • 2
    Publication Date: 2019-09-23
    Description: Ocean Sampling Day was initiated by the EU-funded Micro B3 (Marine Microbial Biodiversity, Bioinformatics, Biotechnology) project to obtain a snapshot of the marine microbial biodiversity and function of the world’s oceans. It is a simultaneous global mega-sequencing campaign aiming to generate the largest standardized microbial data set in a single day. This will be achievable only through the coordinated efforts of an Ocean Sampling Day Consortium, supportive partnerships and networks between sites. This commentary outlines the establishment, function and aims of the Consortium and describes our vision for a sustainable study of marine microbial communities and their embedded functional traits.
    Type: Article , PeerReviewed , info:eu-repo/semantics/article
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  • 3
    Publication Date: 2022-01-31
    Description: Ultraslow spreading ridges account for one-third of the global mid-ocean ridges. Their impact on the diversity and connectivity of benthic deep-sea microbial assemblages is poorly understood, especially for hydrothermally inactive, magma-starved ridges. We investigated bacterial and archaeal diversity in sediments collected from an amagmatic segment (10∘–17∘E) of the Southwest Indian Ridge (SWIR) and in the adjacent northern and southern abyssal zones of similar water depths within one biogeochemical province of the Indian Ocean. Microbial diversity was determined by 16S ribosomal RNA (rRNA) gene sequencing. Our results show significant differences in microbial communities between stations outside and inside the SWIR, which were mostly explained by environmental selection. Community similarity correlated significantly with differences in chlorophyll a content and with the presence of upward porewater fluxes carrying reduced compounds (e.g., ammonia and sulfide), suggesting that trophic resource availability is a main driver for changes in microbial community composition. At the stations in the SWIR axial valley (3,655–4,448 m water depth), microbial communities were enriched in bacterial and archaeal taxa common in organic matter-rich subsurface sediments (e.g., SEEP-SRB1, Dehalococcoida, Atribacteria, and Woesearchaeota) and chemosynthetic environments (mainly Helicobacteraceae). The abyssal stations outside the SWIR communities (3,760–4,869 m water depth) were dominated by OM1 clade, JTB255, Planctomycetaceae, and Rhodospirillaceae. We conclude that ultraslow spreading ridges create a unique environmental setting in sedimented segments without distinct hydrothermal activity, and play an important role in shaping microbial communities and promoting diversity, but also in connectivity among deep-sea habitats.
    Type: Article , PeerReviewed , info:eu-repo/semantics/article
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  • 4
    Publication Date: 2024-02-07
    Description: Seasonal variations in day length and temperature, in combination with dynamic factors such as advection from the North Atlantic, influence primary production and the microbial loop in the Fram Strait. Here, we investigated the seasonal variability of biopolymers, microbial abundance, and microbial composition within the upper 100 m during summer and fall. Flow cytometry revealed a shift in the autotrophic community from picoeukaryotes dominating in summer to a 34-fold increase of Synechococcus by fall. Furthermore, a significant decline in biopolymers concentrations covaried with increasing microbial diversity based on 16S rRNA gene sequencing along with a community shift towards fewer polymer-degrading genera in fall. The seasonal succession in the biopolymer pool and microbes indicates distinct metabolic regimes, with a higher relative abundance of polysaccharide-degrading genera in summer and a higher relative abundance of common taxa in fall. The parallel analysis of DOM and microbial diversity provides an important baseline for microbe-substrate relationships over the seasonal cycle in the Arctic Ocean.
    Type: Article , PeerReviewed
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  • 5
    Publication Date: 2024-04-10
    Description: The long-term dynamics of microbial communities across geographic, hydrographic, and biogeochemical gradients in the Arctic Ocean are largely unknown. To address this, we annually sampled polar, mixed, and Atlantic water masses of the Fram Strait (2015–2019; 5–100 m depth) to assess microbiome composition, substrate concentrations, and oceanographic parameters. Longitude and water depth were the major determinants (~30%) of microbial community variability. Bacterial alpha diversity was highest in lower-photic polar waters. Community composition shifted from west to east, with the prevalence of, for example, Dadabacteriales and Thiotrichales in Arctic- and Atlantic-influenced waters, respectively. Concentrations of dissolved organic carbon peaked in the western, compared to carbohydrates in the chlorophyll-maximum of eastern Fram Strait. Interannual differences due to the time of sampling, which varied between early (June 2016/2018) and late (September 2019) phytoplankton bloom stages, illustrated that phytoplankton composition and resulting availability of labile substrates influence bacterial dynamics. We identified 10 species clusters with stable environmental correlations, representing signature populations of distinct ecosystem states. In context with published metagenomic evidence, our microbial-biogeochemical inventory of a key Arctic region establishes a benchmark to assess ecosystem dynamics and the imprint of climate change.
    Type: Article , PeerReviewed , info:eu-repo/semantics/article
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  • 6
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    PANGAEA
    In:  Supplement to: Braeckman, Ulrike; Janssen, Felix; Lavik, Gaute; Elvert, Marcus; Marchant, Hannah K; Buckner, Caroline; Bienhold, Christina; Wenzhöfer, Frank (2018): Carbon and nitrogen turnover in the Arctic deep sea: in situ benthic community response to diatom and coccolithophorid phytodetritus. Biogeosciences, 15(21), 6537-6557, https://doi.org/10.5194/bg-15-6537-2018
    Publication Date: 2023-03-16
    Description: This is a dataset from an in situ experiment at station S2 from the LTER monitoring site HAUSGARTEN, performed in June-July 2013 during Maria S Merian expedition MSM29. The in situ responses of Arctic deep-sea benthos to input of phytodetritus of a diatom (Thalassiosira sp.) as opposed to a coccolithophorid (Emiliania huxleyi) were investigated in incubation chambers of benthic landers. Using 13C and 15N labelled phytodetritus harvested from cultures of these species, we traced the fate of the respective phytodetritus into different parts of the food web (respiration, assimilation by bacteria and infauna 〉250 µm), in a short (4d) and long (14d) term experiment. The benthic landers were lowered to the sea floor, where they enclosed ~ 20cm of sediment and ~10 cm of overlying water. During respectively 4d and 14d, the temperature and concentrations of O2, DIC, 13C-DIC, NHx, NOx, 15N-NH4, 15N-NOx were measured. Upon recovery of the landers, the sediment was retrieved and subsampled in vertical horizons to measure pigment, TOC and TN, 13C-POC and 15N-PN concentrations, pore water concentrations of DIC, 13C-DIC, NHx, NOx, 15N-NH4 and 15N-NOx and the assimilation of 13C in bacterial fatty acids (iC15:0 and aiC15:0) and in fauna 〉 250 µm
    Keywords: Hausgarten; Long-term Investigation at AWI-Hausgarten off Svalbard
    Type: Dataset
    Format: application/zip, 10 datasets
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  • 7
    Publication Date: 2023-03-13
    Keywords: Acridine Orange Direct Counting (AODC); CHEMECO; Comment; DEPTH, sediment/rock; Event label; HERMES; HERMIONE; Hotspot Ecosystem Research and Mans Impact On European Seas; Hotspot Ecosystem Research on the Margins of European Seas; MEDECO2; MEDECO2-D338-PC-5; MEDECO2-D338-PC-7; Monitoring colonisation processes in chemosynthetic ecosystems; Nile Fan Pockmark Area; Pourquoi Pas ? (2005); Prokaryotes, abundance as single cells; PUC; Push corer
    Type: Dataset
    Format: text/tab-separated-values, 64 data points
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  • 8
    Publication Date: 2023-03-13
    Keywords: Acridine Orange Direct Counting (AODC); CHEMECO; Comment; DEPTH, sediment/rock; Event label; HERMES; HERMIONE; Hotspot Ecosystem Research and Mans Impact On European Seas; Hotspot Ecosystem Research on the Margins of European Seas; Latitude of event; Longitude of event; MEDECO2; MEDECO2-D339-PC-10; MEDECO2-D339-PC-17; Monitoring colonisation processes in chemosynthetic ecosystems; Nile Fan Pockmark Area; Pourquoi Pas ? (2005); Prokaryotes, abundance as single cells; PUC; Push corer
    Type: Dataset
    Format: text/tab-separated-values, 64 data points
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  • 9
    Publication Date: 2023-03-13
    Keywords: Ammonium; Carbon, inorganic, dissolved; CHEMECO; Comment; DEPTH, sediment/rock; Event label; Flow injection analysis (Hall and Aller 1992); HERMES; HERMIONE; Hotspot Ecosystem Research and Mans Impact On European Seas; Hotspot Ecosystem Research on the Margins of European Seas; Ion chromatography; Latitude of event; Longitude of event; MEDECO2; MEDECO2-D338-PC_PW-1; MEDECO2-D338-PC_PW-2; Monitoring colonisation processes in chemosynthetic ecosystems; Nile Fan Pockmark Area; Pourquoi Pas ? (2005); PUC; Push corer; Seawater analysis after Grasshoff; Silicate; Sulfate
    Type: Dataset
    Format: text/tab-separated-values, 160 data points
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  • 10
    Publication Date: 2023-03-13
    Keywords: Ammonium; Carbon, inorganic, dissolved; CHEMECO; Comment; DEPTH, sediment/rock; Event label; Flow injection analysis (Hall and Aller 1992); HERMES; HERMIONE; Hotspot Ecosystem Research and Mans Impact On European Seas; Hotspot Ecosystem Research on the Margins of European Seas; Ion chromatography; Latitude of event; Longitude of event; MEDECO2; MEDECO2-D339-PC_PW-1; MEDECO2-D339-PC_PW-2; Monitoring colonisation processes in chemosynthetic ecosystems; Nile Fan Pockmark Area; Pourquoi Pas ? (2005); PUC; Push corer; Seawater analysis after Grasshoff; Silicate; Sulfate
    Type: Dataset
    Format: text/tab-separated-values, 150 data points
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