ISSN:
1432-1432
Keywords:
Key words: Starvation conditions — Promoter-creating mutations — Heterologous gene transfer — Metabolic evolution — Adaptive mutations
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract. A novel system to study the evolution of transcription signals in heterologous systems under selective starvation conditions is described. It is based on the plasmid-mediated transfer of his biosynthetic genes from Azospirillum brasilense into a heterologous Escherichia coli mutant population lacking histidine biosynthetic ability. We show that under highly selective stressful conditions, genetic changes in the donor plasmid lead to mutated sequences that are efficiently recognized as promoters by the E. coli RNA polymerase.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/PL00013149
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