ISSN:
1617-4623
Keywords:
Erwinia chrysanthemi
;
Glucosidase/xylosidase
;
Xylolytic enzymes
;
Gene cloning
;
bgxA gene sequence
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A β-glucosidase/xylosidase gene from Erwinia chrysanthemi strain D1 was cloned and sequenced. This gene, named bgxA, encodes a ca. 71 kDa protein product which, following removal of the leader peptide, resulted in a ca. 69 kDa mature protein that accumulated in the periplasmic space of E. chrysanthemi strain D1 and Escherichia coli cells expressing the cloned gene. The protein exhibited both β-glucosidase and β-xylosidase activities but gave no detectable activity on xylan or carboxymethyl cellulose. The enzyme was classified as a type 3 glycosyl hydrolase, but was unusual in having a truncated B region at the carboxyl-terminus. Several E. chrysanthemi strains isolated from corn produced the glucosidase/xylosidase activity but not those isolated from dicot plants. However, bgxA marker exchange mutants of strain D1 were not detectably altered in virulence on corn leaves.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00290450
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