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  • 3H-leucine microcentrifuge; Acetone extraction (Turner Designs); Acidic molybdate solution; Alanine; Amino acid, total hydrolysable dissolved; Arabinose; Arctic; Arginine; Aspartic acid; Bacteria; Bacteria, heterotrophic with relatively high DNA content; Bacteria, heterotrophic with relatively low DNA content; Bacterial production; Carbohydrates, dissolved combined; Carbon, organic, dissolved; Chlorophyll a; Coomassie stainable particles; CSP; CTD/Rosette; CTD-RO; Date/Time of event; DEPTH, water; Event label; FACSCalibur flow-cytometer (Becton Dickinson); FRAM; FRontiers in Arctic marine Monitoring; Fucose; Galactosamine; Galactose; Galacturonic acid; Glucosamine; Glucose; Glucuronic acid; Glutamic acid; Glycine; Greenland Sea; Hausgarten; High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000; High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260; High temperature catalytic oxidation (TOC-VCSH); Isoleucine; LATITUDE; Leucine; Leucine incorporation rate; LONGITUDE; Long-term Investigation at AWI-Hausgarten off Svalbard; Mannose/Xylose; Maria S. Merian; Micro-ARC; Microscopy, 20x magnification (Zeiss Axio Scope.A1); MSM77; MSM77_12-1; MSM77_13-1; MSM77_17-1; MSM77_19-1; MSM77_22-1; MSM77_24-1; MSM77_29-1; MSM77_3-1; MSM77_36-1; MSM77_37-1; MSM77_40-1; MSM77_41-1; MSM77_4-3; MSM77_44-1; MSM77_46-4; MSM77_47-1; MSM77_48-1; MSM77_52-1; MSM77_53-3; MSM77_54-1; MSM77_6-1; MSM77_8-1; Nitrogen, total dissolved; organic carbon; Phenylalanine; Phosphorus, organic, dissolved; Phosphorus, total dissolved; Polarstern; PS114; PS114_12-1; PS114_13-2; PS114_16-1; PS114_20-1; PS114_23-1; PS114_25-2; PS114_31-1; PS114_32-2; PS114_33-1; PS114_4-1; PS114_9-1; Rhamnose; Serine; Station label; TEP; Threonine; Transparent exopolymer particles; Transparent exopolymer particles per carbon biomass; Treatment: temperature; Tyrosine; Understanding the links between pelagic microbial ecosystems and organic matter cycling in the changing Arctic (μARC); Valine  (1)
  • 551  (1)
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  • 1
    Publication Date: 2024-01-26
    Description: The discrete biogeochemical measurements from RV Polarstern PS114 and RV Maria S. Merian MSM77 sampled for dissolved organic matter, heterotrophic bacteria and gel particles. The parameters include chlorophyll, DOC/TDN/TDP/DOP, dissolved hydrolysable amino acids, dissolved combined carbohydrates, heterotrophic bacterial abundance and production, gel particles like TEP and CSP. The samples were aquired within 0-100 m of the Greenland Sea between 78°N to 80°N and 2°W to 14°E. The data was collected daily on board PS114 from 16th July 2018-23rd July 2018 and on board MSM77 from 16th September 2018-4th October 2018. The water collection occured using a CTD and laboratory methods varied by parameter. Chlorophyll was extracted using acetone, DOC/TDN was analysed using High-Temperature Catalytic Oxidation (TOC-VCSH), TDP/DOP was analysed colorimetrically using Acidic Molybdate Solution, dissolved hydrolysable amino acids were analysed using High Performal Liquid Chromatography (HPLC) Ortho-phthaldialdehyde Derivatization, dissolved combined carbohydrates were analysed using High Performance Anion Exchange Chromatography (HPAEC) coupled with Pulsed Amperometric Detection (PAD), cell abundance was analysed using flow cytometery, bacterial production was analysed using radioactively labelled 3H-Leucine and apllication of the microcentrifuge method, gel particles were analysed microscopically followed by image analysis. The purpose of data collection was to understand seasonal cycling of organic mater and heterotrophic bacteria dynamics within microbial loop.
    Keywords: 3H-leucine microcentrifuge; Acetone extraction (Turner Designs); Acidic molybdate solution; Alanine; Amino acid, total hydrolysable dissolved; Arabinose; Arctic; Arginine; Aspartic acid; Bacteria; Bacteria, heterotrophic with relatively high DNA content; Bacteria, heterotrophic with relatively low DNA content; Bacterial production; Carbohydrates, dissolved combined; Carbon, organic, dissolved; Chlorophyll a; Coomassie stainable particles; CSP; CTD/Rosette; CTD-RO; Date/Time of event; DEPTH, water; Event label; FACSCalibur flow-cytometer (Becton Dickinson); FRAM; FRontiers in Arctic marine Monitoring; Fucose; Galactosamine; Galactose; Galacturonic acid; Glucosamine; Glucose; Glucuronic acid; Glutamic acid; Glycine; Greenland Sea; Hausgarten; High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000; High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260; High temperature catalytic oxidation (TOC-VCSH); Isoleucine; LATITUDE; Leucine; Leucine incorporation rate; LONGITUDE; Long-term Investigation at AWI-Hausgarten off Svalbard; Mannose/Xylose; Maria S. Merian; Micro-ARC; Microscopy, 20x magnification (Zeiss Axio Scope.A1); MSM77; MSM77_12-1; MSM77_13-1; MSM77_17-1; MSM77_19-1; MSM77_22-1; MSM77_24-1; MSM77_29-1; MSM77_3-1; MSM77_36-1; MSM77_37-1; MSM77_40-1; MSM77_41-1; MSM77_4-3; MSM77_44-1; MSM77_46-4; MSM77_47-1; MSM77_48-1; MSM77_52-1; MSM77_53-3; MSM77_54-1; MSM77_6-1; MSM77_8-1; Nitrogen, total dissolved; organic carbon; Phenylalanine; Phosphorus, organic, dissolved; Phosphorus, total dissolved; Polarstern; PS114; PS114_12-1; PS114_13-2; PS114_16-1; PS114_20-1; PS114_23-1; PS114_25-2; PS114_31-1; PS114_32-2; PS114_33-1; PS114_4-1; PS114_9-1; Rhamnose; Serine; Station label; TEP; Threonine; Transparent exopolymer particles; Transparent exopolymer particles per carbon biomass; Treatment: temperature; Tyrosine; Understanding the links between pelagic microbial ecosystems and organic matter cycling in the changing Arctic (μARC); Valine
    Type: Dataset
    Format: text/tab-separated-values, 6627 data points
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  • 2
    Publication Date: 2021-03-29
    Description: Time-series studies of arctic marine ecosystems are rare. This is not surprising since polar regions arelargely only accessible by means of expensive modern infrastructure and instrumentation. In 1999, theAlfred Wegener Institute, Helmholtz-Centre for Polar and Marine Research (AWI) established the LTER(Long-Term Ecological Research) observatory HAUSGARTEN crossing the Fram Strait at about 79◦N.Multidisciplinary investigations covering all parts of the open-ocean ecosystem are carried out at a totalof 21 permanent sampling sites in water depths ranging between 250 and 5500 m. From the outset,repeated sampling in the water column and at the deep seafloor during regular expeditions in summermonths was complemented by continuous year-round sampling and sensing using autonomous instru-ments in anchored devices (i.e., moorings and free-falling systems). The central HAUSGARTEN stationat 2500 m water depth in the eastern Fram Strait serves as an experimental area for unique biologicalin situ experiments at the seafloor, simulating various scenarios in changing environmental settings.Long-term ecological research at the HAUSGARTEN observatory revealed a number of interesting tem-poral trends in numerous biological variables from the pelagic system to the deep seafloor. Contrary tocommon intuition, the entire ecosystem responded exceptionally fast to environmental changes in theupper water column. Major variations were associated with a Warm-Water-Anomaly evident in sur-face waters in eastern parts of the Fram Strait between 2005 and 2008. However, even after 15 years ofintense time-series work at HAUSGARTEN, we cannot yet predict with complete certainty whether thesetrends indicate lasting alterations due to anthropologically-induced global environmental changes of thesystem, or whether they reflect natural variability on multiyear time-scales, for example, in relation todecadal oscillatory atmospheric processes.
    Keywords: HAUSGARTEN; Arctic Ocean; Deep sea; Natural variability; Anthropogenic impact ; 551
    Language: English
    Type: article , publishedVersion
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