ISSN:
1434-193X
Keywords:
Carbohydrates
;
β-Galactosidase
;
Bacillus circulans
;
Galβ1→4GlcNAc
;
Galβ1→4Gal
;
Enzyme catalysis
;
Chemistry
;
General Chemistry
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Galβ1→4 disaccharide structures are vital core units of the oligosaccharide components of glycoconjugates. β-Galactosidase from Bacillus circulans (E.C.3.2.1.23) catalyses the transfer of galactose from a donor structure such as GalβOpNP to various GlcNAc and galactose derivatives, forming β1→4 linkages. The synthesis of several biologically relevant disaccharides {Galβ1→4GlcNAcαOAll (3), Galβ1→4GlcNAcβOAll (5), Galβ1→4GalαOAll (10), Galβ1→4GalβOAll (12), Galβ1→4GalβSPh (14), Galβ1→4GalβOpNP (16) and the trisaccharide Galβ1→4Galβ1→4GalβOpNP (18)} has been achieved in 30-66 % yield by application of this enzyme.
Type of Medium:
Electronic Resource
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