ISSN:
1573-7276
Keywords:
α4β1
;
fibronectin
;
integrins
;
neural tumor
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Human neural-crest-derived tumor cell lines, including three neuroblastomas, an astrocytoma, a glioblastoma, a rhabdomyosarcoma and a melanoma were screened for the expression of the integrin α4β1 (VLA-4). The neuroblastomas IMR-32 and SK-N-SH, the astrocytoma 131-INI, the glioblastoma Fogerty and the rhabdomyosarcoma TE-671 expressed α4β1 as determined by cytofluorometry and immunoprecipitation. Another neuroblastoma line, LA-N-1, did not express α4β1. Analysis of immunoprecipitated α4β1 showed that the α4 subunit from the various cell types differed in relative molecular weight (M r ). The variability in the observed M r could be accounted for by differences in the levels of N-linked glycosylation. The observed variability in M r did not appear to affect function since intact cells and solubilized α4β1 bound to a synthetic peptide identical in sequence to the CS-1 region of the alternatively spliced IIICS domain of fibronectin, a known α4β1, ligand.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00133564
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