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  • 1
    ISSN: 1432-041X
    Keywords: Key words engrailed ; Thermobia domestica ; Oncopeltus fasciatus ; Dorsal ridge
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Homologues of the Drosophila segment polarity gene engrailed have been cloned from many insect species, as well as other arthropods and non-arthropods. We have cloned partial cDNAs of two engrailed homologues, which we call engrailed-related genes, from the phylogenetically basal insect, Thermobia domestica (Order Thysanura) and possibly as many as four engrailed-related genes from the phylogenetically intermediate insect, Oncopeltus fasciatus (Order Hemiptera). Previous to our findings, only single engrailed-related homologues had been found in phylogenetically intermediate insect species (Tribolium and Schistocerca) and in the crustacean Artemia, while two engrailed-related homologues have been found in more derived orders (Hymenoptera and the engrailed and invected genes of lepidopterans and dipterans). Consequently, we performed a phylogenetic analysis of insect engrailed-related genes to determine whether insects ancestrally had one or two engrailed-related genes. We have found evidence of concerted evolution among engrailed-related paralogues, however, that masks the true phylogenetic history of these genes; the phylogeny may only be decipherable, therefore, by examining the presence or absence of engrailed-specific and invected-specific motifs, which will require cloning the full length cDNAs from more species. In addition, we examined the embryonic expression pattern of the two Thermobia engrailed-related genes; like Drosophila engrailed and invected, they are expressed in very similar patterns, but show one temporal difference in pregnathal segments that correlates with the tentative phylogenetic placement of the genes. Thermobia engrailed-related expression also confirms that the dorsal ridge is an ancient structure in insects.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-041X
    Keywords: Key words Thermobia domestica ; Thysanura ; teashirt ; HOM-C ; Insect evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  During embryogenesis of the fruit fly, Drosophila melanogaster, the homeotic genes are required to specify proper cell fates along the anterior-posterior axis of the embryo. We cloned partial cDNAs of homologues of the Drosophila homeotic gene teashirt and five of the homeotic-complex (HOM-C) genes from the thysanuran insect, Thermobia domestica, and assayed their embryonic expression patterns. The HOM-C genes we examined were labial, Antennapedia, Ultrabithorax, abdominal-A and Abdominal-B. As the expression pattern of these HOM-C genes is largely conserved among insects and as Thermobia is a member of a phylogenetically basal order of insects, we were able to infer their ancestral expression patterns in insects. We compare the expression patterns of the Thermobia HOM-C genes with their expression in Drosophila and other insects and discuss the potential roles these genes may have played in insect evolution. Interestingly, the teashirt homologue shows greater variability between Thermobia and Drosophila than any of the HOM-C genes. In particular, teashirt is not expressed strongly in the Thermobia abdomen, unlike Drosophila teashirt. We propose that teashirt expression has expanded posteriorly in Drosophila and contributed to a homogenization of the Drosophila larval thorax and abdomen.
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  • 3
    ISSN: 1432-2137
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Medicago sativa L.) cultivars are available that produce high-quality forage; however, information is lacking on the consistency of cultivar forage quality over environments and the influence of stand age on quality. Our objectives were to evaluate alfalfa cultivars for consistency of forage quality over time and environments and to test the validity of sampling seeding-year stands for forage quality. We sample eight alfalfa entries (seven cultivars and one experimental germplasm) at bud and flower maturity stages in the seeding year (one harvest) and first production year (two harvests) in six states (Indiana, Kentucky, Minnesota, New York, Washington, and Wisconsin) ANOVA and orthogonal contrast analyses were conducted to assess entry × environment interactions for forage quality. First-cut forage in the first production year had lower forage quality than third-cut forage, and differences between entries were more pronounced at the first cutting. Including seeding-year data in the ANOVA produced a complex location × entry × stand age interaction, indicating that seeding-year data alone were insufficient to characterize alfalfa entries for forage quality. 'Cimarron VR', 'Arrow', and '5432' had the greatest stability for forage quality and could serve as high, medium, and low forage-quality checks, respectively, in forage quality testing trials. 'WL 322 HQ' and 'Pacesetter' often had high quality, but were not stable for forage quality over environments. Correlations between crude protein, acid detergent fiber (ADF), neutral detergent fiber (NDF), and in vitro digestible dry matter were consistent across locations, entries, cutting, and maturities. The high correlation between NDF and ADF (r ≥ 0.97, P ≤ 0.05) suggests that it may not be necessary to use both procedures to predict entry differences in forage quality.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-904X
    Keywords: gp120 ; AIDS-HIV-1 vaccine ; alum adjuvant ; aluminum hydroxide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Purpose. The characterization of recombinant MN gp120/alum vaccine requires the study of the gp120-alum interaction for the successful formulation of an alum-based HIV-1 vaccine. Methods. Several observations suggest that the gpl20-alum interaction is weak, wherein buffer counterions such as phosphate, sulfate, bicarbonate may cause the desorption of gp120 from alum. Comparison of gp120 with other proteins using particle mobility measurements shows that the weak binding of gp120 to alum is not an anomaly. Serum and plasma also cause desorption of gp120 from alum with a half-life of only a few minutes, wherein this half-life may be faster than the in-vivo recruitment of antigen presenting cells to the site of immunization. Results. Immunization of guinea pigs, rabbits and baboons with gp120 formulated in alum or saline demonstrated that alum provides adjuvant activity for gp120, particularly after early immunizations, but the adjuvant effect is attenuated after several boosts. Conclusions. These observations indicate that both the antigen and the adjuvant require optimization together.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Graefe's archive for clinical and experimental ophthalmology 230 (1992), S. 401-405 
    ISSN: 1435-702X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Eighty percent of 30 eyes with chronic cystoid macular edema (CME) after cataract extraction achieved improved visual acuity of three or more lines when treated with 1% indomethacin eye drops. Of these patients 53% demonstrated an “on/off” phenomenon induced by the initiation and cessation of treatment documented by visual acuity measurements and fluorescein angiography. This “on/off” phenomenon suggests that there is a direct relationship between the use of 1% indomethacin eye drops and the resolution of chronic CME after cataract extraction. Previous studies on the treatment of CME with indomethacin have not addressed the use of indomethacin eye drops for chronic CME.
    Type of Medium: Electronic Resource
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