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  • 1
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    PANGAEA
    In:  Supplement to: Nöthig, Eva-Maria; Bracher, Astrid; Engel, Anja; Metfies, Katja; Niehoff, Barbara; Peeken, Ilka; Bauerfeind, Eduard; Cherkasheva, Alexandra; Gäbler-Schwarz, Stefanie; Hardge, Kristin; Kilias, Estelle; Kraft, Angelina; Mebrahtom Kidane, Yohannes; Lalande, Catherine; Piontek, Judith; Thomisch, Karolin; Wurst, Mascha (2015): Summertime plankton ecology in Fram Strait—a compilation of long- and short-term observations. Polar Research, 34, 18 pp, https://doi.org/10.3402/polar.v34.23349
    Publication Date: 2023-06-21
    Description: Between Greenland and Spitsbergen, Fram Strait is a region where cold ice-covered Polar Water exits the Arctic Ocean with the East Greenland Current (EGC) and warm Atlantic Water enters the Arctic Ocean with the West Spitsbergen Current (WSC). In this compilation, we present two different data sets from plankton ecological observations in Fram Strait: (1) long-term measurements of satellite-derived (1998-2012) and in situ chlorophyll a (chl a) measurements (mainly summer cruises, 1991-2012) plus protist compositions (a station in WSC, eight summer cruises, 1998-2011); and (2) short-term measurements of a multidisciplinary approach that includes traditional plankton investigations, remote sensing, zooplankton, microbiological and molecular studies, and biogeochemical analyses carried out during two expeditions in June/July in the years 2010 and 2011. Both summer satellite-derived and in situ chl a concentrations showed slight trends towards higher values in the WSC since 1998 and 1991, respectively. In contrast, no trends were visible in the EGC. The protist composition in the WSC showed differences for the summer months: a dominance of diatoms was replaced by a dominance of Phaeocystis pouchetii and other small pico- and nanoplankton species. The observed differences in eastern Fram Strait were partially due to a warm anomaly in the WSC. Although changes associated with warmer water temperatures were observed, further long-term investigations are needed to distinguish between natural variability and climate change in Fram Strait. Results of two summer studies in 2010 and 2011 revealed the variability in plankton ecology in Fram Strait.
    Keywords: AWI_BioOce; Biological Oceanography @ AWI
    Type: Dataset
    Format: application/zip, 24 datasets
    Location Call Number Limitation Availability
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  • 2
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    TAYLOR & FRANCIS AS
    In:  EPIC3Marine Biology Research, TAYLOR & FRANCIS AS, 10(8), pp. 771-780, ISSN: 1745-1000
    Publication Date: 2019-07-17
    Description: Sequencing of 18S rDNA clone libraries and 454-pyrosequencing are valuable methods used to describe microbial diversity. The massively parallel 454-pyrosequencing generates vast amounts of ribosomal sequence data and has the potential to uncover more organisms, even rare species. However, the relatively short sequence lengths of ~500 bp are suboptimal for taxonomic annotation and phylogenetic analyses. In this study, we assessed the potential of 18S ribosomal clone libraries to complement corresponding 454-pyrosequencing data with near full-length sequence information. This involved a comparison of protist community compositions in five polar samples suggested by 18S rDNA clone libraries with the corresponding community compositions suggested by 454-pyrosequencing. The study was conducted with four Arctic water samples, focusing on the eukaryotic picoplankton (0.4-3 µm), and with one sample collected in the Southern Ocean, examining the whole size spectrum (〉0.4 µm). For all individual samples, the protist community compositions suggested by the two different approaches showed significant similarities. Around 70% of the sequences detected by sequencing of clone libraries were also present in the 454-pyrosequencing data set. However, the clone library sequences reflected only ~20% of the abundant biosphere identified by 454-pyrosequencing and identified ribosomal sequences, that were not detected in the 454-pyrosequencing data sets.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , isiRev
    Format: application/pdf
    Location Call Number Limitation Availability
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