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  • Frontiers  (1)
  • Frontiers Media  (1)
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  • 1
    Publication Date: 2021-04-23
    Description: The development of phosphate sensors suitable for long-term in situ deployments in natural waters, is essential to improve our understanding of the distribution, fluxes, and biogeochemical role of this key nutrient in a changing ocean. Here, we describe the optimization of the molybdenum blue method for in situ work using a lab-on-chip (LOC) analyzer and evaluate its performance in the laboratory and at two contrasting field sites. The in situ performance of the LOC sensor is evaluated using hourly time-series data from a 56-day trial in Southampton Water (UK), as well as a month-long deployment in the subtropical oligotrophic waters of Kaneohe Bay (Hawaii, USA). In Kaneohe Bay, where phosphate concentrations were characteristic of the dry season (0.13 ± 0.03 μM, n = 704), the in situ sensor accuracy was 16 ± 12% and a potential diurnal cycle in phosphate concentrations was observed. In Southampton Water, the sensor data (1.02 ± 0.40 μM, n = 1,267) were accurate to ±0.10 μM relative to discrete reference samples. Hourly in situ monitoring revealed striking tidal and storm derived fluctuations in phosphate concentrations in Southampton Water that would not have been captured via discrete sampling. We show the impact of storms on phosphate concentrations in Southampton Water is modulated by the spring-neap tidal cycle and that the 10-fold decline in phosphate concentrations observed during the later stages of the deployment was consistent with the timing of a spring phytoplankton bloom in the English Channel. Under controlled laboratory conditions in a 250 L tank, the sensor demonstrated an accuracy and precision better than 10% irrespective of the salinity (0–30), turbidity (0–100 NTU), colored dissolved organic matter (CDOM) concentration (0–10 mg/L), and temperature (5–20°C) of the water (0.3–13 μM phosphate) being analyzed. This work demonstrates that the LOC technology is mature enough to quantify the influence of stochastic events on nutrient budgets and to elucidate the role of phosphate in regulating phytoplankton productivity and community composition in estuarine and coastal regimes.
    Type: Article , PeerReviewed , info:eu-repo/semantics/article
    Format: text
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  • 2
    Publication Date: 2022-10-20
    Description: © The Author(s), 2019. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Wang, Z. A., Moustahfid, H., Mueller, A., V., Michel, A. P. M., Mowlem, M., Glazer, B. T., Mooney, T. A., Michaels, W., McQuillan, J. S., Robidart, J. C., Churchill, J., Sourisseau, M., Daniel, A., Schaap, A., Monk, S., Friedman, K., & Brehmer, P. Advancing observation of ocean biogeochemistry, biology, and ecosystems with cost-effective in situ sensing technologies. Frontiers in Marine Science, 6, (2019): 519, doi:10.3389/fmars.2019.00519.
    Description: Advancing our understanding of ocean biogeochemistry, biology, and ecosystems relies on the ability to make observations both in the ocean and at the critical boundaries between the ocean and other earth systems at relevant spatial and temporal scales. After decades of advancement in ocean observing technologies, one of the key remaining challenges is how to cost-effectively make measurements at the increased resolution necessary for illuminating complex system processes and rapidly evolving changes. In recent years, biogeochemical in situ sensors have been emerging that are threefold or more lower in cost than established technologies; the cost reduction for many biological in situ sensors has also been significant, although the absolute costs are still relatively high. Cost savings in these advancements has been driven by miniaturization, new methods of packaging, and lower-cost mass-produced components such as electronics and materials. Recently, field projects have demonstrated the potential for science-quality data collection via large-scale deployments using cost-effective sensors and deployment strategies. In the coming decade, it is envisioned that ocean biogeochemistry and biology observations will be revolutionized by continued innovation in sensors with increasingly low price points and the scale-up of deployments of these in situ sensor technologies. The goal of this study is therefore to: (1) provide a review of existing sensor technologies that are already achieving cost-effectiveness compared with traditional instrumentation, (2) present case studies of cost-effective in situ deployments that can provide insight into methods for bridging observational gaps, (3) identify key challenge areas where progress in cost reduction is lagging, and (4) present a number of potentially transformative directions for future ocean biogeochemical and biological studies using cost-effective technologies and deployment strategies.
    Description: The unpublished work related to iTag and mini-DO sensor was supported by the US National Science Foundation (NSF) (DBI-145559). The US NSF (OCE-1233654), the US National Institute of Standards and Technology (NIST) (60NANB10D024), and the NOAA Sea Grant (2017-R/RCM-51) supported the development of the CHANOS sensor. Part of this work was supported by the European Commission via the STEMM-CCS, AtlantOS, SenseOCEAN, TriAtlas, and Preface projects under the European Union’s Horizon 2020 research and innovation program (Grant Nos. 603521, 654462, 633211, 614141, and 817578), as well as the AWA project (IRD and BMBF; 01DG12073E), and the Blue Belt Initiative (BBI). The work on the LOC nutrients and carbonate sensors was supported by the Autonuts and CarCASS projects, part of the UK Natural Environment Research Council capital program OCEANIDS (NE/P020798/1 and NE/P02081X/1). The work on zooplankton and chlorophyll sensors was co-supported by the ROEC program (Reseau d’Observation en Environnement Côtier 2015–2020) and the European Regional Development Fund (ERDF).
    Keywords: In situ ; Sensor ; OceanObs ; Ocean technology ; EOVs ; Biogeochemistry ; Biology ; Cost effective
    Repository Name: Woods Hole Open Access Server
    Type: Article
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