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  • 1
    Online Resource
    Online Resource
    Cham :Springer International Publishing AG,
    Keywords: Soil microbiology. ; Electronic books.
    Type of Medium: Online Resource
    Pages: 1 online resource (359 pages)
    Edition: 1st ed.
    ISBN: 9783030984151
    Series Statement: Ecological Studies ; v.244
    DDC: 631.469154
    Language: English
    Note: Intro -- Preface -- References -- Contents -- 1: A Brief Introduction to Hot Desert Environments: Climate, Geomorphology, Habitats, and Soils -- 1.1 Desert Climatology -- 1.1.1 Desert Precipitation -- 1.1.2 Drought -- 1.2 Desert Geomorphology -- 1.2.1 Desert Landscapes and Sediments -- 1.3 Desert Habitats -- 1.3.1 Introduction -- 1.3.2 Uplands, Inselbergs, and Rocky Outcrops -- 1.3.3 Drainage Systems -- 1.3.4 Saline Basins, Playas, and River End Points -- 1.3.5 Sand Plains and Sand Dunes -- 1.3.6 Desert Plains and Pediplains -- 1.4 Desert Soils -- 1.4.1 Semi-arid and Arid Soils -- 1.4.2 Introduction to Unconsolidated Porous Media -- 1.4.3 The Desert Pedoderm -- 1.4.4 Impeded Infiltration -- 1.4.5 Organic and Inorganic Carbon in Desert Soils -- 1.4.6 Nitrogen Dynamics in Desert Soils -- 1.5 Conclusion -- References -- 2: Novel Methods for Studying the Structure and Function of Hot Desert Microorganisms and Their Communities -- 2.1 Introduction -- 2.2 Multi-omics and Bioinformatic Advances to Explore Community Structure and Functional Potential -- 2.3 Approaches to Probe Active Members -- 2.4 Metabolomics for Studying Nutrient Exchange -- 2.5 High-Resolution Imaging to Resolve Microbial Environments -- 2.6 Conclusion and Future Perspectives -- References -- 3: Phototrophic Mats of the Desert: The Bacteria of the Biological Soil Crust Community -- 3.1 Introduction: What Constitutes a Biocrust? -- 3.2 Distribution of Biocrusts -- 3.3 Ecosystem Services of Biocrusts -- 3.3.1 Soil Stabilization -- 3.3.2 Water Infiltration -- 3.3.3 Nitrogen Fixation -- 3.3.4 Other Ecosystem Services of Biocrusts -- 3.4 The Bacteria of Biocrusts -- 3.4.1 Bacterial Diversity -- 3.4.2 Bacterial Taxonomy and Function in Biocrusts -- 3.4.2.1 Cyanobacteria -- 3.4.2.2 Structural Cyanobacteria -- 3.4.2.3 Cosmopolitan Cyanobacteria -- 3.4.2.4 Sporadic Cyanobacteria. , 3.4.3 Other Phototrophs -- 3.4.4 Heterotrophic Bacteria -- 3.5 Conclusion -- References -- 4: Microbial Ecology of Hot Desert Soils -- 4.1 Introduction -- 4.2 Hot Desert Edaphic Microbial Community Diversity and Assembly -- 4.2.1 Taxonomic Composition and Assembly Processes of Hot Desert Edaphic Communities -- 4.2.1.1 Prokaryotic Communities -- 4.2.1.2 Microeukaryotic Communities -- 4.2.1.3 The Hot Desert Soil Virosphere -- 4.2.2 Desert Soil Microbial Interactomes: Lessons from Co-occurrence Networks -- 4.3 Functional Properties of Desert Edaphic Communities -- 4.3.1 Desert Edaphic Communities Are Active During Dry Spells -- 4.3.2 The Impact of Water on Arid Soil Microbial Communities -- 4.4 Conclusions and Perspectives: Hot Desert Soil Microorganisms in a Changing World -- References -- 5: Biology of Desert Endolithic Habitats -- 5.1 Introduction -- 5.2 The Endolithic Community -- 5.3 Climate and Macroscopic Drivers of Diversity -- 5.4 The Rock Habitat -- 5.5 Adaptive Strategies and Metabolism -- 5.5.1 Metabolic Pathways -- 5.6 Conclusion and Future Directions -- References -- 6: Journey of a Thousand Miles: The Evolution of Our Understanding of Viruses in Hot Deserts -- 6.1 Introduction -- 6.2 Terrestrial Desert Phage Communities: Soil, Sand, and Rock -- 6.3 Aquatic Desert Phage Communities: Wadi´s, Gueltas, and Springs -- 6.4 Eukaryotic Viruses -- 6.5 Conclusion and Outlook -- References -- 7: C, N, and P Nutrient Cycling in Drylands -- 7.1 Carbon in Drylands Soils -- 7.1.1 Soil Organic Carbon in Drylands Soils -- 7.1.2 Soil Inorganic C in Drylands Soils -- 7.1.3 Biomass Organic C in Dryland Soils -- 7.1.3.1 Vegetation -- 7.1.3.2 Microbial Autotrophs -- 7.1.4 Carbon Sequestration and Loss: The Impact of Abiotic and Biotic Factors -- 7.2 Nitrogen in Dryland Soils -- 7.2.1 Biological Nitrogen Fixation (BNF) as N Input in Drylands. , 7.2.2 Atmospheric N Deposition and N Discharges as N Inputs in Drylands -- 7.2.3 Nitrogen Losses in Drylands -- 7.2.4 Nitrification and Denitrification in Desert Soils -- 7.3 Phosphorus in Dryland Soils -- 7.3.1 P Stocks and Redistribution by Biological Processes in Drylands -- 7.3.2 Abiotic and Biotic Control of P Cycling -- 7.4 Role of Biological Soil Crust in Nutrient (C, N, and P) Cycles -- 7.5 Influence of Hydration-Desiccation Pulses on Nutrient (C, N, and P) Cycles -- 7.6 Impact of Climate Change on Nutrient Cycling -- 7.7 Conclusion -- References -- 8: Diversity and Plant Growth-Promoting Properties of Microbiomes Associated with Plants in Desert Soils -- 8.1 Introduction -- 8.2 Plant-Associated Microorganisms in Hot Desert Biomes -- 8.2.1 Microorganisms Associated with Succulent Plants in Arid Ecosystems -- 8.2.2 Microorganisms Associated with the Rhizosheath-Root Systems of Xerophytic Desert Plants -- 8.2.3 Microorganisms Associated with Halophytes Inhabiting Arid Ecosystems -- 8.2.4 Microorganisms Associated with Oasis Date Palm, a Model Crop in Desert Agro-Ecosystems -- 8.3 PGP Microorganisms Associated with Desert-Adapted Plants as a Biotechnological Resource -- 8.3.1 Where to Select Valuable PGP Microorganisms Against Drought and Their Essential Features -- 8.3.2 How to Isolate Powerful PGP Microorganisms -- 8.3.3 The Functional Roles Exerted by Microorganisms Associated to Desert Plants -- 8.4 Conclusions -- References -- 9: Insights of Extreme Desert Ecology to the Habitats and Habitability of Mars -- 9.1 Introduction -- 9.2 Desert Habitats and Ecosystems on Earth -- 9.2.1 Soils -- 9.2.2 Lithic Substrates -- 9.2.3 Sediments -- 9.2.4 Salts -- 9.3 Insights from Extreme Desert Ecology -- 9.3.1 Trends of Ecological Change in Deserts -- 9.4 Potential Desert Habitats on Mars -- 9.4.1 Soils and Regolith -- 9.4.2 Lithic Substrates. , 9.4.3 Sediments of Potential Paleoplayas/Paleolakes on Mars -- 9.4.4 Salts -- 9.5 Extrapolating Terrestrial Deserts to Mars -- References -- 10: Survival under Stress: Microbial Adaptation in Hot Desert Soils -- 10.1 Introduction: Hot Deserts and Their Challenges to Life -- 10.2 Adaptation to Desiccation and Osmotic Stresses -- 10.2.1 Sporulation -- 10.3 Membrane Modifications -- 10.4 Accumulation of Compatible Solutes -- 10.5 Protein Protection -- 10.6 Biofilm Formation and EPS Production -- 10.7 Adaptation to Oligotrophy -- 10.8 Adaptations to UV Radiation -- 10.9 Temperature Adaptations -- 10.10 Concluding Remarks -- References -- 11: The Response of Soil Microbial Communities to Hydration and Desiccation Cycles in Hot Desert Ecosystems -- 11.1 Introduction to Desert Terminology -- 11.2 Response to Hydration in Desert Ecosystems -- 11.2.1 Rhythm of Plant Primary Production -- 11.2.2 Carbon Dioxide Pulse -- 11.2.3 Nitrogen Pulsing -- 11.3 Bacterial Composition in Desert Soils -- 11.3.1 Biological Soil Crust -- 11.3.2 Topsoil Community Composition -- 11.4 Soil Bacteria Response to Hydration-Desiccation Cycles in Desert Ecosystems -- 11.4.1 The Response of Desert Soil Bacteria to Desiccation -- 11.4.2 The Response of Desert Soil Bacteria to Hydration -- 11.4.3 Pulse Theory and Grime´s Hypothesis -- 11.5 Conclusion -- References -- 12: Hot Desert Microbiology: Perspectives in a Warming World -- References.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Polar biology 23 (2000), S. 644-650 
    ISSN: 1432-2056
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The presence of non-indigenous microbial contaminants resulting from human faecal contamination of old and currently occupied base and field camp sites in South Victoria Land, Antarctica, was assessed by PCR amplification of extracted soil DNA using species-specific PCR primers. Positive controls (samples recovered from the environs of Scott Base, including the sewage outfall) gave strong signals with Escherichia coli primers whereas Clostridium clostridiiforme primers yielded a signal only with the sewage outfall sample. A comparison was made of PCR amplification results from samples from the abandoned Canada Glacier camp site, the Lake Fryxell summer camp site, the Cape Bird Adelie penguin colony and pristine sites from relatively inaccessible regions of the Taylor Valley. Results indicated a possible residual level of E. coli contamination in the abandoned Canada Glacier camp site, but no significant contamination of the currently occupied Lake Fryxell camp site. These data may provide indirect evidence for improved awareness and standards of waste handling and disposal over the past two decades of Dry Valley field research.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-0983
    Keywords: Key words Yeast ; Heterologous gene expression ; Sulfolobus ; Hyperthermophile phosphoglycerate kinase ; Archaea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The gene encoding phosphoglycerate kinase (PGK) from the Archaeon Sulfolobus solfataricus, an organism growing optimally at 87 °C, was inserted into a yeast expression vector under the control of the galactose-inducible GAL1 yeast promoter. This vector was then transformed into a pgk::TRP1 yeast mutant, a strain inhibited for growth on galactose or glucose due to its lack of PGK enzyme. Slow-growing transformants were obtained on galactose plates at 37 °C, but not 28 °C. These transformants contained low levels of transcripts of the heterologous gene and low amounts of thermostable PGK activity. Weak expression of the hyperthermophile gene in yeast, a mesophile, therefore enabled complementation of the yeast pgk defect at 37 °C but not at 28 °C.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-0983
    Keywords: Yeast ; Heterologous gene expression ; Sulfolobus ; Hyperthermophile phosphoglycerate kinase ; Archaea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The gene encoding phosphoglycerate kinase (PGK) from the ArchaeonSulfolobus solfataricus, an organism growing optimally at 87°C, was inserted into a yeast expression vector under the control of the galactose-inducibleGAL1 yeast promoter. This vector was then transformed into apgk::TRP1 yeast mutant, a strain inhibited for growth on galactose or glucose due to its lack of PGK enzyme. Slow-growing transformants were obtained on galactose plates at 37°C, but not 28°C. These transformants contained low levels of transcripts of the heterologous gene and low amounts of thermostable PGK activity. Weak expression of the hyperthermophile gene in yeast a mesophile, therefore enabled complementation of the yeastpgk defect at 37°C but not at 28°C.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Extremophiles 3 (1999), S. 283-291 
    ISSN: 1433-4909
    Keywords: Key words Benzonitrile ; Nitrile ; Nitrilase ; Thermophilic ; Thermostable
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Nitrilase activity was induced in the thermophilic bacterium Bacillus pallidus strain Dac521 by growth on benzonitrile-supplemented minimal medium. The enzyme had a subunit relative molecular mass of 41 kDa but was purified as a complex with a putative GroEL protein (total M r, 600 kDa). The enzyme catalyzed the hydrolysis of aliphatic, aromatic, and heterocyclic nitriles with widely varying k cat/K M values, primarily the result of differences in substrate affinity. Of the nitriles tested, 4-cyanopyridine was hydrolyzed at the fastest rate. Substitution of benzonitrile at the meta or para position either had no effect on catalytic rate or enhanced k cat, while ortho-substitution was strongly inhibitory, probably because of steric hindrance. The effect of catalytic inhibitors was consistent with the presence of active site thiol residues although activity was little affected by putative thiol reagents such as iodoacetate, iodoacetamide, and N-methylmaleimide. Enzymatic activity was constant between pH 6 and 9 with an optimum at pH 7.6. The optimal temperature for activity was 65°C with rapid activity loss at higher temperatures. The purified nitrilase-GroEL complex had the following half-lives of activity: 8.4 h at 50°C, 2.5 h at 60°C, 13 min at 70°C, and less than 3 min at 80°C.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1433-4909
    Keywords: Key wordsThermus filiformis ; α-Amylase ; Extracellular ; Purification ; Properties ; Thermostability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An extracellular α-amylase produced by the thermophilic bacterium Thermus filiformis Ork A2 was purified from cell-free culture supernatant by ion exchange chromatography. The molecular mass was estimated to be 60 000 Da by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The enzyme was rich in both basic and hydrophobic amino acids, presenting the following NH2-terminal amino acid sequence: Thr-Ala-Asp-Leu-Ile-Val-Lys-Ile-Asn-Phe. Amylolytic activity on soluble starch was optimal at pH 5.5–6.0 and 95°C, and the enzyme was stable in the pH range of 4.0–8.0. Calcium enhanced thermostability at temperatures above 80°C, increasing the half-life of activity to more than 8 h at 85°C, 80 min at 90°C, and 19 min at 95°C. Ethylenediaminetetraacetic acid (EDTA) inhibited amylase activity, the inhibition being reversed by the addition of calcium or strontium ions. The α-amylase was also inhibited by copper and mercuric ions, and p-chloromercuribenzoic acid, the latter being reversed in the presence of dithiothreitol. Dithiothreitol and β-mercaptoethanol activated the enzyme. The α-amylase exhibited Michaelis-Menten kinetics for starch, with a K m of 5.0 mg·ml−1 and k cat/K m of 5.2 × 105 ml·mg−1 s−1. Similar values were obtained for amylose, amylopectin, and glycogen. The hydrolysis pattern was similar for maltooligosaccharides and polysaccharides, with maltose being the major hydrolysis product. Glucose and maltotriose were generated as secondary products, although glucose was produced in high levels after a 6-h digestion. To our knowledge this is the first report of the characterization of an α-amylase from a strain of the genus Thermus.
    Type of Medium: Electronic Resource
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