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  • 1
    Electronic Resource
    Electronic Resource
    Autoantibodies in alopecia areata (1984)
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 111 (1984), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2133.1984.tb04055.x
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  • 2
    Electronic Resource
    Electronic Resource
    Genetics of the peroxidase isoenzymes in Petunia (1983)
    Springer
    Theoretical and applied genetics 65 (1983), S. 1-8 
    Details
    ISSN: 1432-2242
    Keywords: Petunia ; Peroxidase ; Differential allelic expression ; Internal site mutations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In P. hybrida and the putative progenitor species P. axillaris s. l. and P. integrifolia s. l. five mobility alleles of the structural gene prxA were found. The five alleles show differential expression during development of tissue and plant, caused by internal site mutations. Analysis of young but not yet flowering plants heterozygous for prxA showed that the allele prxA3 is expressed first, followed by the alleles prxA2, prxA5, prxA4 and prxA1, in that order. In mature leaves of young flowering plants the prxA2 allele has the highest expression level, followed by the alleles prxA3, prxA5, prxA4 and prxA1. In mature and old leaves of old plants the expression level of the alleles prxA2, prxA3, prxA4 and prxA5 is about equal, whereas that of the prxA1 allele is about twice as high. Crossing experiments suggested that between the plants used no external site differences exist that cause clearly detectable changes in developmental allozyme balance. Fast moving anodic peroxidases were detected that have a variable mobility over a considerable distance. The probability that these enzymes are precursors of the PRXa enzymes is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276254
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  • 3
    Electronic Resource
    Electronic Resource
    A gene controlling rate of anthocyanin synthesis and mutation frequency of the gene An1 in Petunia hybrida (1982)
    Springer
    Theoretical and applied genetics 62 (1982), S. 199-203 
    Details
    ISSN: 1432-2242
    Keywords: Anthocyanin synthesis ; Unstable genes ; Petunia hybrida
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The difference in colour intensity between flowers of sporogenic revertants of the white flowering lines W17 and W28 is caused by an incompletely dominant gene Inl. This gene is not linked to the anthocyanin gene Anl. In the dominant state Inl causes a 50% decrease in colour intensity of selfcoloured red flowers. Chromatographic analysis of anthocyanins of plants homozygous recessive or dominant for Inl showed that the same anthocyanins are produced in both genotypes (cyanidin-3-glucoside and cyanidin-3-diglucoside). Anthocyanin synthesis starts at the same stage of development of the flower in both genotypes. When the bud reaches a length of approximately 45 mm, however, anthocyanin synthesis in the Inl Inl line slows down. No influence of the gene Inl on the concentration of dihydroquercetin-7-glucoside in buds and flowers could be observed, which indicates that the influence of Inl on flower colour development is restricted to the last part of the biosynthesis of anthocyanins, i.e. the conversion of dihydroflavonols into anthocyanins. In addition to Inl having a decreasing effect on flower colour intensity, evidence is produced that the gene Inl also influences the reversion frequency of unstable alleles of the gene Anl.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276237
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  • 4
    Electronic Resource
    Electronic Resource
    Genetics of the peroxidase isoenzymes in Petunia (1983)
    Springer
    Theoretical and applied genetics 66 (1983), S. 173-178 
    Details
    ISSN: 1432-2242
    Keywords: Petunia ; Peroxidase ; Differential allelic expression ; Internal and external site mutations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In Petunia four alleles of the gene prxB could be identified by starch gel electrophoresis. Investigation of PRXb allozyme balance during development of tissue and plant of prxB heterozygotes showed differential temporal expression of the four mobility alleles. Analysis of developmental allozyme balance of prxB2/B3 heterozygotes from F1 and F2 progenies indicated the presence of a trans-acting regulatory gene termed Rp1. Segregation for the gene Rp1 indicated monogenic inheritance. Independent segregation of the genes Rp1 and prxB was found. When homozygous and recessive, the gene Rp1 speeds up the start of expression in enzyme activity of the prxB2 allele. The alleles prxB1 and prxB4 are expressed later in development than the alleles prxB2 and prxB3, no matter the Rp1 genotype. The final expression level of the prxB alleles is different for all four alleles, irrespective of the Rp1 genotype.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00265194
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  • 5
    Electronic Resource
    Electronic Resource
    Genetic analysis of instability in Petunia hybrida (1984)
    Springer
    Theoretical and applied genetics 67 (1984), S. 345-355 
    Details
    ISSN: 1432-2242
    Keywords: Unstable mutation ; Transposable elements ; Deletions ; Repair ; Petunia hybrida
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A hypothesis is proposed to interpret the results of crossing experiments with unstable mutants of Petunia hybrida having variegated flowers and showing variation in the colour intensity and the degree of spotting of the corolla. It is postulated that the An1 locus, which is involved in anthocyanin synthesis, is composed of a structural gene with an adjoining regulatory region, the latter in turn comprising two components, viz., the ‘mutator’, responsible for the activation of the structural gene, and the ‘expressor’, controlling the rate of activity. Unstable An1 alleles originate from deletions induced by a transposable element inserted within the regulatory region. Such deletions extend from one of the ends of the inserted element across the adjacent DNA and thus may include parts of the ‘expressor’ and the ‘mutator’. Reversions result from repair of the deletions, the inserted element not necessarily becoming lost in the process.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00272873
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  • 6
    Electronic Resource
    Electronic Resource
    Genetic analysis of instability in Petunia hybrida (1984)
    Springer
    Theoretical and applied genetics 67 (1984), S. 357-366 
    Details
    ISSN: 1432-2242
    Keywords: Unstable mutations ; Transposition ; Anthocyanin ; Repair ; Petunia hybrida
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In crossing experiments with Petunia hybrida, new mutations, some unstable, have been found in descendants of plants having an unstable allele of the anthocyanin gene An1. One of the unstable mutations affecting the new anthocyanin gene An11 was genetically analyzed, and it was subsequently established in which step of anthocyanin synthesis that An11 is involved. The discovery of new, unstable mutations at other loci indicates that in Petunia also a relation exists between unstable mutations and the presence of transposable elements in the genome. It was demonstrated that reverted alleles (an1 +/+) originating from unstable An1 alleles are less stable than the original wild-type allele An1, and that reversions do not increase the chances of occurrence of new, stable or unstable mutations at other loci. These results provide additional arguments in favour of the hypothesis posed in an earlier paper that reversions of unstable An1 alleles are not the result of excision of the inserted transposable element, but are due to the repair of secondary mutations induced by the insert in the regulatory region of the locus. Consequently, a reverted allele still contains the inserted element that may again induce mutations leading to inactivation of An1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00272875
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  • 7
    Electronic Resource
    Electronic Resource
    Genetics of the peroxidase isoenzymes in Petunia (1984)
    Springer
    Theoretical and applied genetics 68 (1984), S. 25-28 
    Details
    ISSN: 1432-2242
    Keywords: Petunia ; Peroxidase ; Differential temporal expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two alleles of the structural gene prxA from Petunia, prxA6 and prxA7, could be identified by their differential temporal expression. The alleles prxA6 and prxA7 code for peroxidases with a similar electrophoretic mobility as the products of the previously described alleles prxA1 and prxA5, respectively. The former two alleles differ in that they have a different temporal expression with regard to the temporal expression of the allele prxA2. Crossing experiments indicated that the mutations involved are (cisacting) internal site mutations. In the case of the allele prxA6, the experiments indicated a difference with respect to the allele prxA1 in responsiveness to the action of a trans-acting factor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00252306
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  • 8
    Electronic Resource
    Electronic Resource
    Genetics of the peroxidase isoenzymes in Petunia (1984)
    Springer
    Theoretical and applied genetics 68 (1984), S. 29-34 
    Details
    ISSN: 1432-2242
    Keywords: Petunia ; Peroxidase isoenzymes ; Flower corolla ; Root ; Gene localization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In root and flower corolla tissue of Petunia several anodic moving peroxidase isoenzymes are present, which cannot be detected in other organs. Alleles of the gene prxF control the presence or absence of several peroxidases that are only present in flower corolla tissue. Alleles of the gene prxG code for two peroxidases that can only be detected in root tissue. In addition to mutations of prxG that cause a change in the electrophoretic mobility of the PRXg enzymes, a mutation was also found that causes the absence of expression in enzyme activity. Crossing experiments indicated that this mutation is located in the gene prxG. Peroxidases encoded by the gene prxH were only found in root tissue. Two alleles of prxH were identified by electrophoretic variation; one allele is responsible for a single band, whereas the other allele could be recognized by a double-banded phenotype. The double-banded PRXh phenotype is suggested to be caused by tandem duplication, followed by mutation in one of the genes. A third prxH allele could be identified by the absence of PRXh activity. The genes prxF, prxG, and prxH were shown to be located on chromosome VII, with the following gene order: prxG-An4-lapB-gpiB-prxH-prxF.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00252307
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  • 9
    Electronic Resource
    Electronic Resource
    Genetics of the peroxidase isoenzymes in Petunia (1984)
    Springer
    Theoretical and applied genetics 68 (1984), S. 265-268 
    Details
    ISSN: 1432-2242
    Keywords: Petunia ; prxA alleles ; Specific peroxidase activities
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Antibodies were raised against the peroxidases encoded by the allele prxA1 to determine the specific activities of the peroxidases encoded by the alleles prxA1, prxA2, prxA3, and prxA5. The results from double diffusion experiments indicated that all peroxidases encoded by the four alleles are antigenically identical. By rocket immuno electrophoresis it was shown that the peroxidases encoded by the alleles prxA1, prxA2, prxA3, and prxA5 have different specific activities. The results presented are discussed in relation to differential expression of the alleles involved.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00266901
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