Publikationsdatum:
2015-05-29
Beschreibung:
Nature Methods 12, 519 (2015). doi:10.1038/nmeth.3370 Authors: Iain C Macaulay, Wilfried Haerty, Parveen Kumar, Yang I Li, Tim Xiaoming Hu, Mabel J Teng, Mubeen Goolam, Nathalie Saurat, Paul Coupland, Lesley M Shirley, Miriam Smith, Niels Van der Aa, Ruby Banerjee, Peter D Ellis, Michael A Quail, Harold P Swerdlow, Magdalena Zernicka-Goetz, Frederick J Livesey, Chris P Ponting & Thierry Voet The simultaneous sequencing of a single cell's genome and transcriptome offers a powerful means to dissect genetic variation and its effect on gene expression. Here we describe G&T-seq, a method for separating and sequencing genomic DNA and full-length mRNA from single cells. By applying G&T-seq to over 220 single cells from mice and humans, we discovered cellular properties that could not be inferred from DNA or RNA sequencing alone.
Print ISSN:
1548-7091
Digitale ISSN:
1548-7105
Thema:
Biologie
,
Medizin
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