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Prolonged Culture of the Voracious Flagellate Peranema in Antioxidant-Containing Media (1966)

ALLEN, JAMES R. ; LEE, JOHN J. ; HUTNER, S. H. ; [et al.]

Oxford, UK : Blackwell Publishing Ltd

The @journal of eukaryotic microbiology 13 (1966), S. 0

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ISSN: 1550-7408

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology

Notes: SYNOPSIS. Peranema trichophorum remained vigorous at least a year in milk-lecithin media and 3 months in a nearly defined autoclavable biphasic agar medium fortified with the fat-soluble antioxidant butylated hydroxytoluene. The only undefined substance in the present “defined” medium is crude soybean lecithin; 0.001% lecithin suffices in the presence of a mixture of long-chain saturated and unsaturated fatty acids. Linoleic acid may be indispensable. Histidine is an absolute requirement as well as a favored substrate. Cholesterol, not ergosterol, satisfied the sterol requirement. Voracity is retained in these media as shown by engorgement on plastic latex particles.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1550-7408.1966.tb01877.x

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