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C-terminal glycine-histidine tagging of the outer membrane protein Iga β of Neisseria gonorrhoeae (1995)

Strauss, Andreas ; Pohlner, Johannes ; Klauser, Thomas ; [et al.]

Oxford, UK : Blackwell Publishing Ltd

FEMS microbiology letters 127 (1995), S. 0

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ISSN: 1574-6968

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology

Notes: Abstract A glycine-histidine tag (Gly3His6) was added to the C-terminus of a fusion protein consisting of the cholera toxin B-subunit (CtxB) and the IgA protease β-domain (Iga β). The aim was to facilitate single-step purification and to create a suitable tool for kinetic and structural studies on Iga β-driven protein translocation across the outer membrane of Gram-negative bacteria. We demonstrate that the glycine-histidine tag does not interfere with the assembly of Iga β in the outer membrane and that the translocator function of the modified Iga β is maintained. The applicability of the new construct for the dissection of the Iga β mediated translocation process and general aspects of C-terminal histidine tagging of outer membrane proteins are discussed.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1574-6968.1995.tb07481.x

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