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  • 1
    Electronic Resource
    Electronic Resource
    The use of indole as a spectrophotometric assay substrate for toluene dioxygenase (1985)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 30 (1985), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Cell extracts of toluene-grown Pseudomonas putida produced a soluble yellow dye during aerobic incubations with indole and NADH. Accumulation of indoxyl in reaction mixtures corresponded with a linear increase in absorbance at 400 nm. The rate of increase in absorbance was shown to be a specific measure of toluene dioxygenase activity. The primary product of toluene oxidation, cis-toluene dihydrodiol, inhibited dioxygenase activity in cell extracts containing no detectable activity of cis-toluene dihydrodiol dehydrogenase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1985.tb01017.x
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