ISSN:
1432-1424
Keywords:
cornea
;
A-type potassium channel
;
epithelium
;
patch clamp
;
perforated patch
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Summary Ionic currents from freshly dissociated rabbit corneal endothelial cells were examined using patch-clamp technology and a perforated patch technique. Whole-cell current recordings revealed a transient outward K+-selective current that was blockable in a dose-dependent manner by 4-aminopyridine (4-AP) and quinidine. This current is similar to the ‘A’-type current present in many excitable cells and is the first reported instance of such a current in any epithelial cell type. In addition to the transient current, an outwardly rectifying nonselective cation current was also observed. This current is also blocked by quinidine. To examine the possible role of these currents in the stromal volume regulatory function of the endothelium, corneas were perfused under a specular microscope with a glutathionebicarbonate Ringer's solution (GBR) or GBR plus either 1 mM quinidine or 10 mM 4-AP. For quinidine perfusions, control corneas swelled at a rate of 6 μm/hr, while quinidine-perfused corneas swelled at a rate of 48 μm/hr. For 4-AP perfusions, control corneas deswelled at a rate of −2 μm/hr, while 4-AP perfused corneas swelled at a rate of 24 μm/hr. One possible mechanism of the stromal swelling induced by these K+ channel blockers may be the result of loss of the K+ recycling pathway necessary for proper Na+/K+ ATPase function.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00231885
