ISSN:
1617-4623
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Mutants of E. coli specifically deficient for the enzyme altronic hydrolyase have been isolated. These strains are unable to metabolize galacturonate but still normally grow on glucuronate; inducibility for the other galacturonate-induced enzymes is not modified. The position of these mutations called “uxaA” locus in relation to the argG, tolC and metC markers was established both by sexual crosses and by P1 transduction; evidence is presented that uxaA is located between argG and tolC. Biochemical characterization of uxaA mutants using thermosensible revertants or mutants was achieved by means of thermal inactivation and kinetic parameters determination. Experimental results strongly suggest that uxaA locus is the structural gene of the altronic hydrolyase enzyme.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00333569