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Micropropagation of Hemidesmus indicus (L.) R. Br. through axillary bud culture (1996)

Patnaik, J. ; Debata, B.K.

Springer

Plant cell reports 15 (1996), S. 427-430

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ISSN: 1432-203X

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: ABSTRACT. A procedure for rapid in vitro propagation of the aromatic and medicinal plant Hemidesmus indicus (L.) R.Br. (Family Asclepiadeceae) from nodal explants is described. The highest shoot multiplication rate of 8.2±0.4 shoots/explant with a 95% frequency was achieved in 5 weeks culture period on Murashige and Skoog medium supplemented with 1.15 μM kinetin and 0.054 μM α-naphthaleneacetic acid. Excised shoots were rooted on the same basal medium supplemented with 1.15 μM kinetin and 7.35 μM indole-3-butyric acid. Shoots derived from subcultures exhibited better rooting response than those from primary cultures. After a hardening phase of 2 weeks, there was a 70% transplantation success in the field.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s002990050047

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