ISSN:
1432-0983
Keywords:
Phycomyces
;
pyrF
;
pyrG
;
Complementation
;
Recombination
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A total of seven pyrimidine auxotrophs of Phycomyces were isolated from among 5-fluoroorotate acid (5-FOA)-resistant mutants. They were classified by complementation into two groups. A representative mutant strain belonging to one group was deficient in orotate phosphoribosyl transferase (OPRTase; EC 2.4.2.10) activity; the mutant strain belonging to the second group was deficient in orotidine-5′-monophosphate decarboxylase (OMPdecase; EC 4.1.1.23). These mutants are defective in the genes pyrF and pyrG respectively. The results from random spore analysis, tetrad analysis, and gene-centromere distances showed that these two markers are located in linkage group VI, with pyrG being a proximal marker and pyrF a distal one.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00351715
