ISSN:
1432-0983
Keywords:
Yeast plasmid
;
Transformation
;
pKD1
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The circular plasmid pKD1 (or 1.6 μm DNA) has recently been isolated from Kluyveromyces drosophilarum. This plasmid appears to have a functional organization analogous to that of the 2 μ DNA of Saccharomyces cerevisiae, although the respective nucleotide sequences show little homology. pKD1 can be transferred to Kluyveromyces lactis where it is replicated stably. Using recombinant molecules derived from pKDl, a practical transformation system has been developed for Kluyveromyces lactis, with an efficiency and stability comparable to the 2 μ-based Saccharomyces cerevisiae transformation system.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00436877