ISSN:
1432-0614
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Summary Yeast cells producing the growth hormone somatomedin C (SMC) were constructed and applied in the immobilized form continuously for a period of over 10 days in a flow-through bioreactor. The construction of the MFα1-SMC fusion vector p336/1 is given as well as the results of the influence of various nutrients effecting hormone production. Immobilization of the transformed yeast cells is described and their application in a continuous bioreactor system. This study demonstrates the feasibility of a longterm and high-level hormone production by immobilized transformed yeast. The SMC productivities of free cells in batch and immobilized cells under continuous conditions were 0.2–0.3 and 0.5–0.6 mg per g wet cells and day, respectively.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00250444
