ISSN:
1573-2568
Keywords:
SODIUM BUTYRATE
;
ALKALINE PHOSPHATASE ACTIVITY
;
INTESTINAL EPITHELIAL CELLS
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Sodium butyrate is a well-recognizeddifferentiating agent inducing alkaline phosphataseactivity, one of the epithelial differentiation markers.When IEC6 cells, a nontransformed, small intestinalepithelial cell line, were cultured with butyrate, thissubstrate induced alkaline phosphate activity in a time-and dose-dependent fashion. However, the type ofisoenzyme involved was a liver-type, not anintestinal-type. Electron microscopy revealed that the inducedactivity was strictly localized in the cytosol and noton the plasma membrane. However, disaccharidaseactivities, another kind of differentiation marker, were also enhanced by sodium butyrate. In addition,the positive cells demonstrating the presence ofalkaline phosphatase activity were preferentiallyobserved in tubular structures. These data show thatbutyrate-induced alkaline phosphatase activity is closelyassociated with differentiation-like phenomena in IEC6cells, although the type of isoenzyme and cellularlocalization of the activity are different from thoseobserved in mucosa.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1018807524075
