ISSN:
1573-0603
Keywords:
in vitro
;
fluorescent dyes
;
cell culture
;
cytotoxicity
;
L929
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary A semi-quantitative procedure is described for measuring cell viability after short-term exposure to a test substance using a monolayer culture. Test substances are placed in direct contact with cell monolayers for various time intervals. The substances are removed and the monolayers are incubated in the presence of fluorescein diacetate. Monolayers are viewed under a fluorescent microscope and the percentage of fluorescing (viable) cells is estimated. The method is suitable for examining cytotoxic effects at short times of exposure and for discriminating between test substances that give similar, low toxicity endpoints in standard 24-h assays.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02388271
