ISSN:
0173-0835
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
We have studied the electrophoretic mobility of purified human C reactive protein (CRP) in a gradient from pH 3.0 to 9.0 produced by the titration curve method. The results suggest that the major species has a pI at pH 6.3 ± 0.2. From pH 7.2 to 8.5, CRP was at its isoelectric point and did not migrate from the application trough. From pH 4.0 to 5.0, CRP produced a hazy, diffuse pattern most probably due to instability in the region close to the isoelectric point of the acid-denatured form of the molecule. Below pH 4 CRP resolubilized to assume heterogenic anionic forms.
Additional Material:
1 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elps.1150040414
