Keywords:
Proteins -- Analysis -- Laboratory manuals.
;
Nuclear magnetic resonance spectroscopy -- Laboratory manuals.
;
Electronic books.
Type of Medium:
Online Resource
Pages:
1 online resource (915 pages)
Edition:
1st ed.
ISBN:
9780080515298
URL:
https://ebookcentral.proquest.com/lib/geomar/detail.action?docID=582023
DDC:
572/.636
Language:
English
Note:
Front cover -- Title page -- Copyright page -- Preface -- Preface to the First Edition -- Acknowledgements -- Table of Contents -- 1 Classical NMR Spectroscopy -- 1.1 Nuclear Magnetism -- 1.2 The Bloch Equations -- 1.3 The One-Pulse NMR Experiment -- 1.4 Linewidth -- 1.5 Chemical Shift -- 1.6 Scalar Coupling and Limitations of the Bloch Equations -- References -- 2 Theoretical Description of NMR Spectroscopy -- 2.1 Postulates of Quantum Mechanics -- 2.2 The Density Matrix -- 2.3 Pulses and Rotation Operators -- 2.4 Quantum Mechanical NMR Spectroscopy -- 2.5 Quantum Mechanics of Multispin Systems -- 2.6 Coherence -- 2.7 Product Operator Formalism -- 2.8 Averaging of the Spin Hamiltonians and Residual Interactions -- References -- 3 Experimental Aspects of NMR Spectroscopy -- 3.1 NMR Instrumentation -- 3.2 Data Acquisition -- 3.3 Data Processing -- 3.4 Pulse Techniques -- 3.5 Spin Decoupling -- 3.6 B0 Field Gradients -- 3.7 Water Suppression Techniques -- 3.8 One-Dimensional 1H NMR Spectroscopy -- References -- 4 Multidimensional NMR Spectroscopy -- 4.1 Two-Dimensional NMR Spectroscopy -- 4.2 Coherence Transfer and Mixing -- 4.3 Coherence Selection, Phase Cycling, and Field Gradients -- 4.4 Resolution and Sensitivity -- 4.5 Three- and Four-Dimensional NMR Spectroscopy -- References -- 5 Relaxation and Dynamic Processes -- 5.1 Introduction and Survey of Theoretical Approaches -- 5.2 The Master Equation -- 5.3 Spectral Density Functions -- 5.4 Relaxation Mechanisms -- 5.5 Nuclear Overhauser Effect -- 5.6 Chemical Exchange Effects in NMR Spectroscopy -- References -- 6 Experimental 1H NMR Methods -- 6.1 Assessment of the 1D 1H Spectrum -- 6.2 COSY-Type Experiments -- 6.3 Multiple-Quantum Filtered COSY -- 6.4 Multiple-Quantum Spectroscopy -- 6.5 TOCSY -- 6.6 Cross-Relaxation NMR Experiments -- 6.7 1H 3D Experiments -- References.
,
7 Heteronuclear NMR Experiments -- 7.1 Heteronuclear Correlation NMR Spectroscopy -- 7.2 Heteronuclear-Edited NMR Spectroscopy -- 7.3 13C-13C Correlations: The HCCH-COSY and HCCH-TOCSY Experiments -- 7.4 3D Triple-Resonance Experiments -- 7.5 Measurement of Scalar Coupling Constants -- 7.6 Measurement of Residual Dipolar Coupling Constants -- References -- 8 Experimental NMR Relaxation Methods -- 8.1 Pulse Sequences and Experimental Methods -- 8.2 Picosecond-Nanosecond Dynamics -- 8.3 Microsecond-Second Dynamics -- References -- 9 Larger Proteins and Molecular Interactions -- 9.1 Larger Proteins -- 9.2 Intermolecular Interactions -- 9.3 Methods for Rapid Data Acquisition -- References -- 10 Sequential Assignment, Structure Determination, and Other Applications -- 10.1 Resonance Assignment Strategies -- 10.2 Three-Dimensional Solution Structures -- 10.3 Conclusion -- References -- Table of Symbols -- List of Figures -- List of Tables -- Suggested Reading -- Biomolecular NMR Spectroscopy -- NMR Spectroscopy -- Quantum Mechanics -- Index -- Spin-1/2 Product Operator Equations -- Table of Constants.
