Note: Front Cover -- Methods in Cell Biology, Volume 30 -- Copyright Page -- Contents -- Preface -- Contributors -- Chapter 1. Image Fidelity: Characterizing the Imaging Tranfer Function -- I. Introduction -- II. The Concept of a Linear, Shift-lnvarient (LSI) System -- III. Characterizing LSI Systems with Sinusoids -- IV. Characterizing the Fluorescence Imaging System -- V. The Contrast Modulation Transfer Function (CMTF) -- VI. Relating the CMTF and the OTF -- VII. Implications -- VIII. The Analysis of the Z-Axis -- IX. Summary -- References -- Chapter 2. Validation of an Imaging System -- I. Shading Correction -- II. Linearity -- III. Geometric Correction and Registration -- IV. Modulation Transfer -- V. Sampling Frequency -- VI. Summary -- References -- Chapter 3. Imaging of Unresolved Objects, Superresolution, and Precision of Distance Measurement, with Video Microscopy -- I. Visualizing Objects Narrower than the Resolution Limit of the Light Microscope -- II. Diffraction Patterns of Very Narrow Objects -- III. Superresolution -- IV. Positional Information and Lateral Setting Accuracy -- V. Ultrathin Optical Sectioning and Axial Setting Accuracy -- VI. Other Factors Affecting the Choice of Pixel Dimensions, Calibra- tion, and Choice of Magnification -- References -- Chapter 4. Fluorescent Standards -- I. Introduction -- II. System Standards -- III. Analytical Calibration Standards -- IV. Concluding Statement -- References -- Chapter 5. Fluorescent Indicators of Ion Concentrations -- I. Introduction -- II. Proton Indicators -- III. Sodium Indicators -- IV. Chloride Indicators -- V. Calcium Indicators -- VI. Prospects and Conclusions -- References -- Chapter 6. Fluorescence Ratio Imaging Microscopy -- I. Introduction -- II. Basic Principles of Fluorescence Ratio Imaging -- III. Requirements for a Fluorescence Ratio Imaging Microscope System. , IV. Validation of the Fluorescence Ratio Imaging Microscope System -- V. Measurements Using Fluorescence Ratio Imaging -- VI. Conclusions and Prospectus -- References -- Chapter 7. Fluorescent Indicators of Membrane Potential: Microspectrofluorometry and Imaging -- I. Introduction -- II. Microspectrofluorometry with Nernstian Dyes -- III. Imaging with Charge-Shift Dyes -- IV. Discussion -- References -- Chapter 8. Resonance Energy Transfer Microscopy -- I. Introduction -- II. Fluorescence Theory -- III. Techniques of Measurement in the Microscope -- IV. Experimental Parameters Measured in the Microscope -- V. Biological Applications -- VI. Problems -- VII. Conclusions -- References -- Chapter 9. Total Internal Reflection Fluorescence Microscopy -- I. Introduction -- II. Theory -- III. Optical Configuration for a Microscope -- IV. Applications of TIRF -- V. Conclusions -- References -- Chapter 10. Designing, Building, and Using a Fluorescence Recovery after Photobleaching Instrument -- 1. Introduction -- II. Designing and Building a Spot FPR Instrument -- III. Artifacts of FPR -- IV. Beyond Spot FPR-Obtaining Spatial Information -- V. Conclusion -- References -- Chapter 11. Interpretation of Fluorescence Correlation Spectroscopy and Photobleaching Recovery in Terms of Molecular Interactions -- I. Introduction -- II. Characteristic Responses -- III. Technical Issues -- IV. Measurement of Aggregation of Fluorophores by FCS -- V. Interaction Systems -- VI. Summary -- References -- Chapter 12. Fluorescence Polarization Microscopy -- I. Introduction -- II. Theory -- III. Experiment -- References -- Chapter 13. Fluorescence Microscopy in Three Dimensions -- I. Introduction -- II. Image Formation in Three Dimensions -- III. Removal of Out-of-Focus Information -- IV. Synthetic Projection and Stereographic Imaging -- V. Experimental Considerations. , VI . Display -- VII. Experimental Results for Imaging Chromosomes -- VIII. Summary -- References -- Chapter 14. Three Dimensional Confocal Fluorescence Microscopy -- I. Introduction -- II. Confocal Microscopy -- III. Confocal Imaging Results -- IV. Discussion -- References -- Chapter 15. Emission of Fluorescence at an Interface -- I. Introduction -- II. Theory -- Ill. Graphical Results -- IV. Consequences for Experiments -- V. Conclusions -- References -- Chapter 16. Fluorescence Labeling and Microscopy of DNA -- I. Introduction -- II. Labeling Strategies -- III. Measurements -- IV. Measurement Techniques -- V. New Directions in FDIM -- References -- Chapter 17. Multiple Spectral Parameter Imaging -- I. Introduction -- II. Practical Aspects of Multiple Spectral Parameter Imaging -- References -- Index -- Content of Resent Volumes.