Note: Front Cover -- Methods in Cell Biology, Volume 29 -- Copyright Page -- Contents -- Contributors -- Preface -- Chapter 1. Fluorescent Analog Cytochemistry: Tracing Functional Protein Components in Living Cells -- I. Introduction -- II. Equipping the Laboratory for Fluorescent Analog Cytochemistry -- III. Preparation of Fluorescent Analogs -- IV. Assays of Fluorescent Analogs and Preparations for Microinjection -- V. Delivery of the Conjugates and Handling of Living Cells -- VI. Data Collection and Interpretation -- VII. Prospectus -- References -- Chapter 2. Fluorescent Analogs of Peptides and Hormones -- I. Introduction -- II. Designing a Fluorescent Analog -- III. Characterization of Fluorescent Analogs -- IV. Examples of the Use of Fluorescent Analogs of Hormones and Peptides -- References -- Chapter 3. Fluorescent Analogs of Toxins -- I. Introduction -- II. Examples of Fluorescent Toxins -- III. Selection, Preparation, and Utilization of Fluorescent Toxin Analogs -- IV. Use of Fluorescent Toxin Analogs -- V. Requirements for Successful Application of Toxin Analogs -- References -- Chapter 4. Preparation of Fluorescently Labeled Dextrans and Ficolls -- I. Introduction -- II. Methods -- III. Further Considerations in the Use of Fluorescent Dextrans and Ficolls -- References -- Chapter 5. A Fluorescent Derivative of Ceramide: Physical Properties and Use in Studying the Golgi Apparatus of Animal Cells -- I. Introduction -- II. Synthesis and Purification of C6-NBD-ceramide -- III. Use of Resonance Energy Transfer (RET) to Study C6-NBD-ceramide in Liposomes -- IV. Properties of C6-NBD-ceramide in Cells -- V. Prospectus -- References -- Chapter 6. Fluorescent Labeling of Cell Surfaces -- I. Introduction -- II. Direct Chemical Labels of Cell Surfaces -- III. Fluorescent Ligands as Labels of Cell Surfaces -- References. , Chapter 7. Fluorescent Labeling of Mitochondria -- I. Introduction -- II. Unique Property of Mitochondria: High Membrane Potential -- III. Fluorescent Dyes with Delocalized Positive Charges -- IV. Rhodamine 123 as a Conditional Supravital Dye -- V. Methods of Labeling and Quantitation -- VI. Mitochondrial Localization -- VII. Mitochondrial Morphology -- VIII. Mitochondrial Motility and Distribution -- IX. Monitoring Mitochondrial Membrane Potential -- X. Monitoring Mitochondrial Total Electrochemical Gradient -- XI. Flow Cytometry, Cell Sorting, and Other Applications -- References -- Chapter 8. Fluorescent Labeling of Endoplasmic Reticulum -- I. Introduction -- II. Materials -- III. Labeling Procedures -- IV. Analysis of the Staining -- V. Summary -- References -- Chapter 9. Fluorescent Labeling of Endocytic Compartments -- I. Introduction -- II. Fluorescent Probes -- III. Identifying Compartments of the Endocytic Pathway -- References -- Chapter 10. Incorporation of Macromolecules into Living Cells -- I. Introduction -- II. Strategies for Loading Living Cells -- III. Selected Techniques for Loading Living Cells -- IV. Evaluation and Analysis of Loaded Cells -- References -- Chapter 11. Hapten-Mediated Immunocytochemistry: The Use of Fluorescent and Nonfluorescent Haptens for the Study of Cytoskeletal Dynamics in Living Cells -- I. Introduction -- II. Advantages of Hapten-Mediated Immunocytochemistry -- III. Preparation of Reagents -- IV. Application of Hapten-Mediated Immunocytochemistry -- V. Conclusion and Future Outlook -- VI. Technical Appendix -- References -- Chapter 12. Culturing Cells on the Mircroscope Stage -- I. Introduction -- II. Requirements of the Microscope Culture System -- III. Cell Culture Chambers -- IV. Microscope Incubators -- References -- Chapter 13. Basic Fluorescence Microscopy -- I. Introduction -- II. Microscope Design. , III. Practical Issues of Epiillumination Fluorescence Microscopy -- IV. Basic Setup of an Epiillumination Fluorescence Microscope -- References -- Chapter 14. Quantitative Fluorescence Microscopy Using Photomultiplier Tubes and Imaging Detectors -- I. Introduction -- II. Accessories for Quantitative Fluorescence Analysis -- III. Detectors for Quantitative Fluorescence Microscopy -- References -- Chapter 15. Characteristics of Low Light Level Television Cameras -- I. Introduction -- II. Requirements for a Low Light Level Video Camera -- III. Criteria for Camera Selection and Evaluation -- IV. Performance Characteristics of Existing Low Light Level Cameras -- V. Future Developments -- References -- Chapter 16. Solid-State Imagers for Microscopy -- I. The History of Solid-State Imagers -- II. Theory of CCD Imagers -- III. CCD Camera Implementations -- IV. CCD Performance -- V. Quantitative Imaging with CCDs -- VI. Future Trends in Solid-State Detector Development -- VII. Summary -- References -- Index -- Contents of Recent Volumes.