Description: A simulated flooding experiment was conducted to evaluate the effects of seasonal flooding on the plant Salix triandroides from the Dongting Lake wetlands in China. The morphology, photosynthetic activity, and anatomy of cuttings in three water conditions (−40 cm, water level 40 cm below soil surface; 0 cm, water level 0 cm at the soil surface; and 40 cm, water level 40 cm above soil surface) and two lights conditions (full sunlight and 10% sunlight) were measured. Plants had a higher survival ratio and biomass accumulation in full sunlight than in 10% sunlight when the water level was −40 and 0 cm, but there was no difference between these parameters in cuttings grown under the two light conditions in the 40 cm water treatment. In full sunlight, a lower survival ratio and reduced biomass were observed with increasing water level. The same trend was also seen for survival ratio in 10% sunlight. However, there was no difference in biomass among the three water levels in 10% sunlight, except for leaf weight. Branch height, leaf number, adventitious root length, and adventitious root number were different in the three water levels and two light conditions. In water levels of −40 and 0 cm, plants had lower chlorophyll contents in full sunlight than in 10% sunlight. In full sunlight, there was no difference in chlorophyll content between the water levels, while in 10% sunlight, lower chlorophyll content was observed in −40 cm than in 0 cm water. Photosynthetic rate, stomatal conductance, and transpiration rate decreased, but water-use efficiency increased in reduced light at all three water levels. Additionally, plants had higher porosity in 40 cm water than in −40 and 0 cm conditions. Based on the reduced plant growth in the 10% sunlight condition and decreased survival in the 40 cm water level, we conclude that low light significantly decreased plant acclimation to incomplete submergence and that high water levels induced dormancy in the cuttings. Therefore, the height of cuttings used for forestation or reforestation is an important consideration for mitigating the negative effects of seasonal flooding on the survival and growth of S. triandroides in Dongting Lake wetlands.