Publication Date:
2016-08-23
Description:
Purpose The aim of this work was to investigate whether hyperpolarized 13 C, 15 N 2 -urea can be used as an imaging marker of renal injury in renal unilateral ischemic reperfusion injury (IRI), given that urea is correlated with the renal osmotic gradient, which describes the renal function. Methods Hyperpolarized three-dimensional balanced steady-state 13 C magnetic resonance imaging (MRI) experiments alongside kidney function parameters and quantitative polymerase chain reaction measurements were performed in rats subjected to unilateral renal ischemia for 60-minute and 24-hour reperfusion. Results We revealed a significant reduction in the intrarenal gradient in the ischemic kidney in agreement with cortical injury markers neutrophil gelatinase-associated lipocalin and kidney injury molecule 1, as well as functional kidney parameters. Conclusion Hyperpolarized functional 13 C, 15 N 2 urea MRI can be used to successfully detect changes in the intrarenal urea gradient post-IRI, thereby enabling in vivo monitoring of the intrarenal functional status in the rat kidney. Magn Reson Med, 2016. © 2016 International Society for Magnetic Resonance in Medicine
Print ISSN:
0740-3194
Electronic ISSN:
1522-2594
Topics:
Medicine
