Publication Date:
2012-02-29
Description:
Lipidic phase membrane protein serial femtosecond crystallography Nature Methods 9, 263 (2012). doi:10.1038/nmeth.1867 Authors: Linda C Johansson, David Arnlund, Thomas A White, Gergely Katona, Daniel P DePonte, Uwe Weierstall, R Bruce Doak, Robert L Shoeman, Lukas Lomb, Erik Malmerberg, Jan Davidsson, Karol Nass, Mengning Liang, Jakob Andreasson, Andrew Aquila, Saša Bajt, Miriam Barthelmess, Anton Barty, Michael J Bogan, Christoph Bostedt, John D Bozek, Carl Caleman, Ryan Coffee, Nicola Coppola, Tomas Ekeberg, Sascha W Epp, Benjamin Erk, Holger Fleckenstein, Lutz Foucar, Heinz Graafsma, Lars Gumprecht, Janos Hajdu, Christina Y Hampton, Robert Hartmann, Andreas Hartmann, Günter Hauser, Helmut Hirsemann, Peter Holl, Mark S Hunter, Stephan Kassemeyer, Nils Kimmel, Richard A Kirian, Filipe R N C Maia, Stefano Marchesini, Andrew V Martin, Christian Reich, Daniel Rolles, Benedikt Rudek, Artem Rudenko, Ilme Schlichting, Joachim Schulz, M Marvin Seibert, Raymond G Sierra, Heike Soltau, Dmitri Starodub, Francesco Stellato, Stephan Stern, Lothar Strüder, Nicusor Timneanu, Joachim Ullrich, Weixiao Y Wahlgren, Xiaoyu Wang, Georg Weidenspointner, Cornelia Wunderer, Petra Fromme, Henry N Chapman, John C H Spence & Richard Neutze X-ray free electron laser (X-FEL)-based serial femtosecond crystallography is an emerging method with potential to rapidly advance the challenging field of membrane protein structural biology. Here we recorded interpretable diffraction data from micrometer-sized lipidic sponge phase crystals of the Blastochloris viridis photosynthetic reaction center delivered into an X-FEL beam using a sponge phase micro-jet.
Print ISSN:
1548-7091
Electronic ISSN:
1548-7105
Topics:
Biology
,
Medicine