Publication Date:
2022-05-25
Description:
Author Posting. © The Author(s), 2009. This is the author's version of the work. It is posted here by permission of Blackwell and Society for Applied Microbiology for personal use, not for redistribution. The definitive version was published in Environmental Microbiology Reports 2 (2010): 236-242, doi:10.1111/j.1758-2229.2009.00097.x.
Description:
Corroborative data collected from 16S rRNA clone libraries, intergenic transcribed spacer (ITS) region clone libraries, and 16S rRNA hypervariable region tag pyrosequencing demonstrate microdiversity within single-species archaeal biofilms of the Lost City Hydrothermal Field. Both 16S rRNA clone libraries and pyrosequencing of the V6 hypervariable region show that Lost City Methanosarcinales (LCMS) biofilms are dominated by a single sequence, but the pyrosequencing dataset also reveals the presence of an additional 1654 rare sequences. Clone libraries constructed with DNA spanning the V6 hypervariable region and ITS show that multiple ITS sequences are associated with the same dominant V6 sequence. Furthermore, ITS variability differed among three chimney samples, and the sample with the highest ITS diversity also contained the highest V6 diversity as measured by clone libraries as well as tag pyrosequencing. These results indicate that the extensive microdiversity detected in V6 tag sequences is an underestimate of genetic diversity within the archaeal biofilms.
Description:
This research was supported by the W.M. Keck Foundation to MLS, the NASA Astrobiology Institute through the Carnegie Institution for Science to JAB and through the MBL to MLS.
Repository Name:
Woods Hole Open Access Server
Type:
Preprint
Format:
application/pdf
