ISSN:
1432-1335
Keywords:
Key words Tropomyosin
;
TM5/TM30nm
;
Isoform
;
Transformation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract We cloned a full-length rat TM5/TM30nm cDNA. Using this cDNA as a probe, we demonstrated that expression of TM5/TM30nm mRNA was higher in the tumorigenic rat fibroblastic cell lines SR-3Y1-2 and fos-SR-3Y1-202 than in the normal cell line 3Y1. High expression of TM5/TM30nm protein in SR-3Y1-2 and fos-SR-3Y1-202 cells was also detected by Western blot analysis using anti-TM5/TM30nm antiserum. In addition, the cellular localization of this protein differed between 3Y1 cells and tumorigenic ones. These findings suggest that TM5/TM30nm is involved in malignant transformation of rat fibroblastic cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01438309