Publikationsdatum:
2014-12-20
Beschreibung:
The Anoxybacillus sp. SK 3–4, previously isolated from a hot spring, was screened for its heavy metals resistance (Al 3+ , Mn 2+ , Cu 2+ , Co 2+ , Zn 2+ , and Ni 2+ ) and the strain was found to be most resistant to aluminum. Significant growth of the strain was observed when it was grown in medium containing aluminum (200 mg L −1 –800 mg L −1 ) with relative growth rates ranging between 77% and 100%. A gene encoding the aluminum resistance protein (accession number: WP_021095658.1) was found in genome of strain SK 3–4, which revealed high sequence identity (〉95%) to its homologues from Anoxybacillus species. Sequence comparisons with two functionally characterized aluminum resistance proteins, namely G2alt and ALU1-P, showed 97% and 81% of sequence identity, respectively. Four putative metal binding sites were detected in SK 3–4 aluminum resistance protein and G2alt at same amino acid residue positions of 186, 195, 198, and 201. Strain SK 3–4 was found to be able to remove aluminum from aqueous solution. This study demonstrated that Anoxybacillus sp. SK 3–4 could be applied in the treatment of aluminum contaminated wastewater.
Print ISSN:
0233-111X
Digitale ISSN:
1521-4028
Thema:
Biologie
