In:
PeerJ, PeerJ, Vol. 4 ( 2016-03-31), p. e1889-
Abstract:
Background. Resveratrol is a natural polyphenol that exhibits anti-inflammatory effects. The aim of this study was to investigate the effects of resveratrol treatment on epithelium-derived cytokines and epithelial apoptosis in a murine model of atopic dermatitis-like lesions. Material and Methods. Atopic dermatitis-like lesions were induced in BALB/c mice by repeated application of 2,4-dinitrofluorobenzene to shaved dorsal skin. Twenty-one BALB/c mice were divided into three groups: group I (control), group II (vehicle control), and group III (resveratrol). Systemic resveratrol (30 mg/kg/day) was administered repeatedly during the 6th week of the experiment. After the mice had been sacrificed, skin tissues were examined histologically for epithelial thickness. Epithelial apoptosis (caspase-3) and epithelium-derived cytokines [interleukin (IL)-25, IL-33, and thymic stromal lymphopoietin (TSLP)] were evaluated immunohistochemically. Results. Epithelial thickness and the numbers of IL-25, IL-33, TSLP and caspase-3-positive cells were significantly higher in group II compared to group I mice. There was significant improvement in epithelial thickness in group III compared with group II mice ( p 〈 0.05). The numbers of IL-25, IL-33, and TSLP-positive cells in the epithelium were lower in group III than in group II mice ( p 〈 0.05). The number of caspase-3-positive cells, as an indicator of apoptosis, in the epithelium was significantly lower in group III than in group II mice ( p 〈 0.05). Conclusion. Treatment with resveratrol was effective at ameliorating histological changes and inflammation by acting on epithelium-derived cytokines and epithelial apoptosis.
Type of Medium:
Online Resource
ISSN:
2167-8359
DOI:
10.7717/peerj.1889/fig-1
DOI:
10.7717/peerj.1889/fig-2
DOI:
10.7717/peerj.1889/fig-3
DOI:
10.7717/peerj.1889/fig-4
DOI:
10.7717/peerj.1889/fig-5
DOI:
10.7717/peerj.1889/fig-6
DOI:
10.7717/peerj.1889/table-1
DOI:
10.7717/peerj.1889/table-2
DOI:
10.7717/peerj.1889/supp-1
DOI:
10.7717/peerj.1889/supp-2
DOI:
10.7717/peerj.1889/supp-3
DOI:
10.7717/peerj.1889/supp-4
Language:
English
Publisher:
PeerJ
Publication Date:
2016
detail.hit.zdb_id:
2703241-3