In:
PeerJ, PeerJ, Vol. 9 ( 2021-11-15), p. e12398-
Abstract:
Due to the global COVID-19 pandemic, there is a need to screen for novel compounds with antiviral activity against SARS-COV-2. Here we compared chemical composition and the in vitro anti- SARS-COV-2 activity of two different Ulva sp. crude ulvan extracts: one obtained by an HCl-based and another one by ammonium oxalate-based (AOx) extraction protocols. The composition of the crude extracts was analyzed and their antiviral activity was assessed in a cytopathic effect reduction assay using Vero E6 cells. We show that the extraction protocols have a significant impact on the chemical composition, anti- SARS-COV-2 activity, and cytotoxicity of these ulvan extracts. The ulvan extract based on the AOx protocol had a higher average molecular weight, higher charge, and 11.3-fold higher antiviral activity than HCl-based extract. Our results strongly suggest that further bioassay-guided investigation into bioactivity of compounds found in Ulva sp. ulvan extracts could lead to the discovery of novel anti-SARS-CoV-2 antivirals.
Type of Medium:
Online Resource
ISSN:
2167-8359
DOI:
10.7717/peerj.12398/fig-1
DOI:
10.7717/peerj.12398/fig-2
DOI:
10.7717/peerj.12398/fig-3
DOI:
10.7717/peerj.12398/fig-4
DOI:
10.7717/peerj.12398/fig-5
DOI:
10.7717/peerj.12398/fig-6
DOI:
10.7717/peerj.12398/table-1
DOI:
10.7717/peerj.12398/supp-1
DOI:
10.7717/peerj.12398/supp-2
DOI:
10.7717/peerj.12398/supp-3
DOI:
10.7717/peerj.12398/supp-4
DOI:
10.7717/peerj.12398/supp-5
Language:
English
Publisher:
PeerJ
Publication Date:
2021
detail.hit.zdb_id:
2703241-3