In:
Advanced Materials Research, Trans Tech Publications, Ltd., Vol. 781-784 ( 2013-9), p. 1403-1409
Abstract:
Nattokinase is a potent fibrinolytic enzyme. The composition of the medium for nattokinase production was optimized respectively. Effect of glucose concentration on nattokinase activity and optimal glucose concentration in fermentation process were investigated. L 9 (3 3 ) orthogonal array design was applied to investigate the effects of Na 2 HPO 4 to NaH 2 PO 4 ratio, CaCl 2 , MgSO 4 on nattokinase activity and search for the optimal concentrations of the three inorganic salts. The results showed that Na 2 HPO 4 /NaH 2 PO 4 was the main factor to affect nattokinase activity, then was CaCl 2 and MgSO 4 in turn. The optimum concentrations of liquid fermentation medium components were as followed: glucose 1% , Na 2 HPO 4 to NaH 2 PO 4 ratio 0.4% : 0.1% , CaCl 2 0.01% and MgSO 4 0.1%. Under the optimal conditions, the nattokinase activity was equivalent to 871.56 urokinase IU/mL. To mask the smelly flavor, the nattokinase powders were encapsulated. The research will be helpful to the development of nattokinase products.
Type of Medium:
Online Resource
ISSN:
1662-8985
DOI:
10.4028/www.scientific.net/AMR.781-784
DOI:
10.4028/www.scientific.net/AMR.781-784.1403
Language:
Unknown
Publisher:
Trans Tech Publications, Ltd.
Publication Date:
2013
detail.hit.zdb_id:
2265002-7
