In:
Frontiers in Aging Neuroscience, Frontiers Media SA, Vol. 14 ( 2022-10-28)
Abstract:
Astrocytic Ca 2+ transients are essential for astrocyte integration into neural circuits. These Ca 2+ transients are primarily sequestered in subcellular domains, including primary branches, branchlets and leaflets, and endfeet. In previous studies, it suggests that aging causes functional defects in astrocytes. Until now, it was unclear whether and how aging affects astrocytic Ca 2+ transients at subcellular domains. In this study, we combined a genetically encoded Ca 2+ sensor (GCaMP6f) and in vivo two-photon Ca 2+ imaging to determine changes in Ca 2+ transients within astrocytic subcellular domains during brain aging. We showed that aging increased Ca 2+ transients in astrocytic primary branches, higher-order branchlets, and terminal leaflets. However, Ca 2+ transients decreased within astrocytic endfeet during brain aging, which could be caused by the decreased expressions of Aquaporin-4 (AQP4). In addition, aging-induced changes of Ca 2+ transient types were heterogeneous within astrocytic subcellular domains. These results demonstrate that the astrocytic Ca 2+ transients within subcellular domains are affected by aging differently. This finding contributes to a better understanding of the physiological role of astrocytes in aging-induced neural circuit degeneration.
Type of Medium:
Online Resource
ISSN:
1663-4365
DOI:
10.3389/fnagi.2022.1029533
DOI:
10.3389/fnagi.2022.1029533.s001
DOI:
10.3389/fnagi.2022.1029533.s002
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2022
detail.hit.zdb_id:
2558898-9
