In:
Frontiers in Molecular Biosciences, Frontiers Media SA, Vol. 9 ( 2022-5-2)
Abstract:
Serine tRNAs (tRNA Ser ) are frequently overexpressed in tumors and associated with poor prognosis and increased risk of recurrence in breast cancer. Impairment of tRNA biogenesis and abundance also impacts proteome homeostasis, and activates protein quality control systems. Herein, we aimed at testing whether increasing tRNA Ser abundance could foster tumor establishment through activation of the UPR. In order to do so, firstly we confirmed that the expression of tRNA-Ser-AGA-2-1 [hereafter tRNA Ser (AGA)] was upregulated by 1.79-fold in Stage I NSCLC tumors when compared to normal adjacent tissue. To study the impact of tRNA Ser (AGA) in early stage tumorigenesis, we induced its upregulation in a non-tumoral bronchial cell line, BEAS-2B. Upregulation of this tRNA increased cellular proliferation and protein synthesis rate, driven by eIF2 α dephosphorylation and ATF4 activation downstream of PERK signaling. Futhermore, tRNA Ser (AGA) enhanced transformation potential in vitro , and promoted the establishment of slow growing tumors with aggressive features in nude mice. Our work highlights the importance of studying tRNA deregulation on early stage tumorigenesis, as they may be potential malignancy and aggressiveness biomarkers.
Type of Medium:
Online Resource
ISSN:
2296-889X
DOI:
10.3389/fmolb.2022.809985
DOI:
10.3389/fmolb.2022.809985.s001
DOI:
10.3389/fmolb.2022.809985.s002
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2022
detail.hit.zdb_id:
2814330-9
