In:
Frontiers in Microbiology, Frontiers Media SA, Vol. 14 ( 2023-4-5)
Abstract:
Although restriction-modification systems are found in both Eubacterial and Archaeal kingdoms, comparatively less is known about patterns of DNA methylation and genome defense systems in archaea. Here we report the complete closed genome sequence and methylome analysis of Methanococcus aeolicus PL15/H p , a strain of the CO 2 -reducing methanogenic archaeon and a commercial source for Mae I, Mae II, and Mae III restriction endonucleases. The M. aeolicus PL15/H p genome consists of a 1.68 megabase circular chromosome predicted to contain 1,615 protein coding genes and 38 tRNAs. A combination of methylome sequencing, homology-based genome annotation, and recombinant gene expression identified five restriction-modification systems encoded by this organism, including the methyltransferase and site-specific endonuclease of Mae III. The Mae III restriction endonuclease was recombinantly expressed, purified and shown to have site-specific DNA cleavage activity in vitro .
Type of Medium:
Online Resource
ISSN:
1664-302X
DOI:
10.3389/fmicb.2023.1112734
DOI:
10.3389/fmicb.2023.1112734.s001
DOI:
10.3389/fmicb.2023.1112734.s002
DOI:
10.3389/fmicb.2023.1112734.s003
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2023
detail.hit.zdb_id:
2587354-4
