In:
Frontiers in Microbiology, Frontiers Media SA, Vol. 13 ( 2022-12-19)
Abstract:
Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method using only one type of enzyme that can amplify DNA with high specificity, efficiency and rapidity under isothermal conditions. Chips for Complicated Infection Detection (CCID) is based on LAMP. This study translate CCID into clinical application and evaluate its diagnostic value for pneumonia. Methods Eighty one older patients with pneumonia were prospectively enrolled from January 1 to July 23, 2021, and 57 sputum/airway secretion and 35 bronchoalveolar lavage fluid samples were collected and analyzed by CCID and conventional microbiological tests (CMTs). Samples were collected, transported, monitored, and managed by a multidisciplinary team using a sample management information system. Results CCID turnaround time was 50 min, and the detection limit was 500 copies/reaction. The percentage of positive samples was significantly higher using CCID than CMTs, especially for Klebsiella pneumoniae (odds ratio [OR], 9.0; 95% confidence interval [CI] , 1.1–70.5; p & lt; 0.05), Enterococcus faecalis (OR, ∞; p & lt; 0.01), Stenotrophomonas maltophilia (OR, ∞; p & lt; 0.01), fungi (OR, 26.0; 95% CI, 3.6–190.0; p & lt; 0.01), and viruses (CCID only; p & lt; 0.01). In addition, the percentage of positive results was significantly higher using CCID than CMTs in patients who used antibiotics for more than 3 days (91.9% vs. 64.9%; p & lt; 0.01). Analyzing clinical impact, 55 cases (59.8%) benefited from CCID. Conclusion CCID allows the rapid and accurate detection of pneumonia in older patients. Moreover, this technique is less affected by previous antibiotic treatment and can improve patient care.
Type of Medium:
Online Resource
ISSN:
1664-302X
DOI:
10.3389/fmicb.2022.1048997
DOI:
10.3389/fmicb.2022.1048997.s001
DOI:
10.3389/fmicb.2022.1048997.s002
DOI:
10.3389/fmicb.2022.1048997.s003
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2022
detail.hit.zdb_id:
2587354-4
