In:
Frontiers in Immunology, Frontiers Media SA, Vol. 14 ( 2023-4-11)
Abstract:
Huge progress has been made in understanding the biology of innate lymphoid cells (ILC) by adopting several well-known concepts in T cell biology. As such, flow cytometry gating strategies and markers, such as CD90, have been applied to indentify ILC. Here, we report that most non-NK intestinal ILC have a high expression of CD90 as expected, but surprisingly a sub-population of cells exhibit low or even no expression of this marker. CD90-negative and CD90-low CD127 + ILC were present amongst all ILC subsets in the gut. The frequency of CD90-negative and CD90-low CD127 + ILC was dependent on stimulatory cues in vitro and enhanced by dysbiosis in vivo . CD90-negative and CD90-low CD127 + ILC were a potential source of IL-13, IFNγ and IL-17A at steady state and upon dysbiosis- and dextran sulphate sodium-elicited colitis. Hence, this study reveals that, contrary to expectations, CD90 is not constitutively expressed by functional ILC in the gut.
Type of Medium:
Online Resource
ISSN:
1664-3224
DOI:
10.3389/fimmu.2023.1113735
DOI:
10.3389/fimmu.2023.1113735.s001
DOI:
10.3389/fimmu.2023.1113735.s002
DOI:
10.3389/fimmu.2023.1113735.s003
DOI:
10.3389/fimmu.2023.1113735.s004
DOI:
10.3389/fimmu.2023.1113735.s005
DOI:
10.3389/fimmu.2023.1113735.s006
DOI:
10.3389/fimmu.2023.1113735.s007
DOI:
10.3389/fimmu.2023.1113735.s008
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2023
detail.hit.zdb_id:
2606827-8