In:
Research, Society and Development, Research, Society and Development, Vol. 9, No. 12 ( 2020-12-13), p. e3991210699-
Abstract:
Objective: Evaluate the miRNA-15 expression profile involved in cellular apoptotic regulation factors. Methodology: We used the H818308 Asian strain of ZIKV without neurological damage. The inoculations occurred in human embryonic kidney cells (HEK-293). After inoculation, samples were extracted for RT-qPCR quantification of viral RNA and miR-15. The level of activation of caspases 1, 3/7 and 8 of cells was performed using chemofluorescence. Results: The ZIKV infection alters the expression of genes and their regulators, affecting several cellular physiological processes such as apoptosis. Conclusion: Therefore, it is important to emphasize that renal progenitor cells (HEK-293) are susceptible to VZIK infection. The genetic deregulation resulting from infection directly affects important cellular processes such as apoptosis from the disordered miRNA-15 expression during the infection period.
Type of Medium:
Online Resource
ISSN:
2525-3409
DOI:
10.33448/rsd-v9i12.10699
Language:
Unknown
Publisher:
Research, Society and Development
Publication Date:
2020