In:
Science & Technology Development Journal - Health Sciences, Viet Nam National University Ho Chi Minh City, ( 2021)
Abstract:
Objective: To isolate and identification Aggregatibacter actinomicetemcomitans from clinical subgingival plaques of periodontitis patients. Methods: Selective media was prepared by adding 200 ug/mL of vancomycin and 10 U/mL of bacitracin, 5% sheep blood to Wilkins Chalgren Anaerobic agar. Subgingival samples collected from patients with moderate to severe periodontitis aged 18- 35 were used to recover Aa. The colony was then identified by the MALDI-TOF method and PCR analysis. Results: The Aa was successfully cultured and stored from a clinical sample of the 12th 25 years-old patient who had moderate aggressive periodontitis. Conclusion: Aa is a major putative oral pathogen and concerned with other systemic diseases. This pure bacterial culture will be helpful for other experimental studies in dental and medical areas.
Type of Medium:
Online Resource
ISSN:
2734-9446
DOI:
10.32508/stdjhs.v2i2.467
Language:
Unknown
Publisher:
Viet Nam National University Ho Chi Minh City
Publication Date:
2021