In:
Regenerative Medicine, Future Medicine Ltd, Vol. 9, No. 3 ( 2014-05), p. 309-326
Abstract:
Aim: To develop a biomimetic polymeric injectable hydrogel that can support nucleus pulposus (NP) regeneration. Materials & methods: Natural polymer-based hydrogels were synthesized using fibrinogen (FBG) and hyaluronic acid (HA), conjugated by a novel two-step procedure. Bovine NP cells were cultured in FBG–HA conjugate-based 3D beads in vitro and in a nucleotomized organ culture model. Results: FBG–HA conjugate-based hydrogels prepared with 235 KDa HA at a FBG/HA w/w ratio of 17:1 showed superior gel stability and mechanical properties and markedly increased glycosaminoglycan synthesis compared with a FBG/HA mixture-based hydrogels or fibrin gels. Gene-expression levels of NP markers were maintained in vitro. In organ culture, NP cells seeded in FBG–HA conjugate-based hydrogels showed better integration with native NP tissue compared with fibrin gels. Moreover, FBG–HA conjugate-based hydrogels restored compressive stiffness and disc height after nucleotomy under dynamic load. Conclusion: Specific FBG–HA conjugate-based hydrogels may be suitable as injectable materials for minimally invasive, biological NP regeneration.
Type of Medium:
Online Resource
ISSN:
1746-0751
,
1746-076X
Language:
English
Publisher:
Future Medicine Ltd
Publication Date:
2014
