In:
The Open Microbiology Journal, Bentham Science Publishers Ltd., Vol. 8, No. 1 ( 2014-9-18), p. 78-86
Abstract:
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) protein analysis, automated ribotyping, and phenotypic tests (e.g., cell morphology, gas production from glucose, growth and acid production on homofermemtative-heterofermentative differential (HHD) agar medium, sugar fermentation patterns) were used to identify 23 lactic acid bacteria (LAB) isolated from fermented cereal foods available in Abidjan, Côte d’Ivoire. Pediococcus acidilactici (56.5%), Lactobacillus fermentum (30.4%), L . salivarius (4.3%), P . pentosaceus (4.3%) and L. plantarum subsp. plantarum (4.3%) were the species and subspecies identified. Protein based identification was confirmed by automated ribotyping for selected isolates and was similar to that provided by the phenotypic characterization. MALDI-TOF MS protein analysis provided a high level of discrimination among the isolates and could be used for the rapid screening of LAB starter cultures.
Type of Medium:
Online Resource
ISSN:
1874-2858
DOI:
10.2174/1874285801408010078
Language:
English
Publisher:
Bentham Science Publishers Ltd.
Publication Date:
2014
detail.hit.zdb_id:
2395971-X
